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Anti-BAD Antibody (N-Terminus, clone BYC001) IHC-plus™ LS-B3293

Note: This antibody replaces LS-C3887


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular BAD Antibodies

Anti-BAD Antibody (phospho-Ser118) LS-C97416
Rabbit Polyclonal to Human BAD
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-BAD Antibody (N-Terminus, clone BYC001) IHC-plus™ LS-B3293
Mouse Monoclonal [clone BYC001] (IgG) to Mouse BAD
Mouse, Human
IHC - Paraffin, Western blot, Immunoprecipitation
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone BYC001] (IgG) to Mouse BAD
Mouse, Human
IHC - Paraffin, Western blot, Immunoprecipitation


Mouse BAD
Mouse, Human (tested or 100% immunogen sequence identity)
IgG Monoclonal [BYC001]
Protein G purified


  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • Immunoprecipitation

Specificity and Use

BAD antibody was raised against full-length murine Bad containing a N-terminal His6-tag.
Recognizes human Bad, Mr 23kD, and mouse Bad, Mr 31kD. Occasionally a Mr 14kD protein is detected in human lysates.
Immunohistochemistry: LS-B3293 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3293 was determined to be 10 ug/ml. Western Blot: Proteins were visualized using IgG (HRP) goat anti-mouse and a chemiluminescence detection system. The endogenous level of Bad in most lysates is below the detectable limits of this antibody. Immunoprecipitation: 4 ug immunoprecipitates Bad which was subsequently detected using anti-phospho Bad.


0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide. Sourced in Ascites.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About BAD

Q92934 NM_004322 NP_004313.1

BAD Antibody, BBC2 Antibody, Bcl-2-like protein 8 Antibody, BCL2-binding component 6 Antibody, BCL2L8 Antibody, BBC6 Antibody, BCL-X/BCL-2 binding protein Antibody, Bcl-2-binding component 6 Antibody, Bcl2 antagonist of cell death Antibody, BCL2-binding protein Antibody, Bcl2-L-8 Antibody

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax.


Anti-BAD antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3293 concentration 10 ug/ml.


Anti-BAD antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3293 concentration 10 ug/ml.

Requested From: United States
Date Requested: 10/26/2016

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