Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Human (tested or 100% immunogen sequence identity)
Monkey, Horse (at least 90% immunogen sequence identity)
IHC - Paraffin (1:1000 - 1:5000)
IHC - Frozen
Western blot (1:1000 - 1:2000)
Immunoprecipitation (1:50 - 1:200)
Specificity and Use
BAD antibody was raised against a synthetic peptide corresponding to amino acids 87-103 (GEEPSPFRGRSRSAPPN) of human Bad, GenBank no. NP_004313.1. Amino acids 88-103 of the peptide immunogen are 93% conserved in mouse and rat Bad. Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey, Marmoset, Cat, Horse (94%); Mouse, Rat, Bat, Hamster (88%); Dog, Elephant, Panda (82%).
Antiserum, 0.05% sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
BAD Antibody, BBC2 Antibody, Bcl-2-like protein 8 Antibody, BCL2-binding component 6 Antibody, BCL2L8 Antibody, BBC6 Antibody, BCL-X/BCL-2 binding protein Antibody, Bcl-2-binding component 6 Antibody, Bcl2 antagonist of cell death Antibody, BCL2-binding protein Antibody, Bcl2-L-8 Antibody
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax.