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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BAD Antibody (aa100‑149) LS‑C117830
BAD antibody LS-C117830 is an unconjugated rabbit polyclonal antibody to BAD from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C117830-50
50 µl
Unavailable
LS-C117830-100
100 µl
Unavailable

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Product Description

BAD antibody LS-C117830 is an unconjugated rabbit polyclonal antibody to BAD from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
About BAD
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax. Q92934 NM_004322 NP_004313.1

BAD Antibody, BBC2 Antibody, Bcl-2-like protein 8 Antibody, BCL2-binding component 6 Antibody, BCL2L8 Antibody, BBC6 Antibody, BCL-X/BCL-2 binding protein Antibody, Bcl-2-binding component 6 Antibody, Bcl2 antagonist of cell death Antibody, BCL2-binding protein Antibody, Bcl2-L-8 Antibody


Specifications

Target
Human BAD
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Immunogen
BAD antibody was raised against synthetic peptide from human BAD (aa100-149).
Epitope
aa100-149
Specificity
BAD (Ab-134) Antibody detects endogenous levels of total BAD protein.
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of extracts from mouse liver cells, using BAD (Ab-134) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from mouse liver cells, using BAD (Ab-134) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from mouse liver cells, using BAD (Ab-134) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from mouse liver cells, using BAD (Ab-134) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from mouse liver cells, using BAD (Ab-134) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from mouse liver cells, using BAD (Ab-134) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from mouse liver cells, using BAD (Ab-134) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from mouse liver cells, using BAD (Ab-134) Antibody. The lane on the right is treated with the synthesized peptide.


Requested From: 
Date Requested: 6/19/2018

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