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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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B2M / Beta 2 Microglobulin Antibody (clone B2M‑01) IHC‑plus™ LS‑B1854
Note: This antibody replaces LS-C45844
Beta 2 Microglobulin antibody LS-B1854 is an unconjugated mouse monoclonal antibody to human Beta 2 Microglobulin (B2M). Validated for ELISA, Flow, ICC, IHC, Inhb, IP, RIA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1854-50
50 µg (1 mg/ml)
Unavailable

Popular B2M / Beta 2 Microglobulin Products

Immunohistochemistry of Rabbit anti-Beta-2-Microglobulin Antibody. Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Left panel: isotype control, Right panel: 2 microglobulin antibody at 1 ug/ml for 20 min at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: 2 microglobulin is cell membrane (and to lesser amount cytoplasmic compartment). Staining: Beta-2-Microglobulin as brown with diaminobenzidine and with a hematoxylin purple counterstain.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Anti-B2M antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2200 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Anti-B2M antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2249 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
Anti-B2M antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2426 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry, ELISA

Product Description

Beta 2 Microglobulin antibody LS-B1854 is an unconjugated mouse monoclonal antibody to human Beta 2 Microglobulin (B2M). Validated for ELISA, Flow, ICC, IHC, Inhb, IP, RIA and WB. Tested on 20 paraffin-embedded human tissues.
About B2M / Beta 2 Microglobulin
B2M / Beta 2 Microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia. P61769 NM_004048 NP_004039.1

B2M Antibody, Beta 2 Microglobulin Antibody, Beta-2-microglobulin Antibody, Beta-2-microglobin Antibody


Specifications

Target
Human B2M / Beta 2 Microglobulin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [B2M-01]
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, PE, HRP.
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ICC
  • Western blot (2 - 4 µg/ml)
  • Immunoprecipitation
  • Flow Cytometry (1 µg/ml)
  • ELISA
  • Inhibition
  • Radioimmunoassay
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified human B2M / Beta-2-Microglobulin.
Specificity
Purified human beta2-microglobulin
Usage
Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody,
Presentation
PBS, 15 mM sodium azide, approx., pH 7.4.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-B2M antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1854 concentration 10 ug/ml.
Anti-B2M antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1854 concentration 10 ug/ml.

Immunohistochemistry

Anti-B2M antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1854 concentration 10 ug/ml.
Anti-B2M antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1854 concentration 10 ug/ml.

Immunohistochemistry

Anti-B2M antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1854 concentration 10 ug/ml.
Anti-B2M antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1854 concentration 10 ug/ml.

Immunohistochemistry

Anti-B2M antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1854 concentration 10 ug/ml.
Anti-B2M antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1854 concentration 10 ug/ml.


Requested From: 
Date Requested: 6/18/2018

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