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Anti-B2M / Beta 2 Microglobulin Antibody (clone 2213) IHC-plus™ LS-B2249

Note: This antibody replaces LS-C11402

Ordering

Wt. Vol. Conc. Price
- 50 µl 1.1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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B2M / Beta 2 Microglobulin Antibody LS-B2249 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-B2M antibody IHC of human skin.
Immunohistochemistry
Anti-B2M antibody IHC of human liver.

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Mouse Monoclonal [clone 2213] (IgG1) to Human B2M / Beta 2 Microglobulin
Human
IHC - Paraffin, ELISA
Unconjugated

Details

Human B2M / Beta 2 Microglobulin
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [2213]
Protein A purified

Applications

IHC - Paraffin (5 µg/ml), ELISA

Specificity and Use

B2M / Beta 2 Microglobulin antibody was raised against highly pure beta-2-microglobulin from human urine.
Specific for human beta 2 microglobulin. B2M is the beta chain of MHC class I molecules. The precursor protein has a molecular weight of approximately 14 kD and is 119 amino acids in length.
Immunohistochemistry: LS-B2249 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2249 was determined to be 5 ug/ml.

Packaging

PBS, pH 8.0, 0.05% sodium azide, 10% glycerol.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About B2M / Beta 2 Microglobulin

P61769 NM_004048 NP_004039.1

B2M Antibody, Beta 2 Microglobulin Antibody, Beta-2-microglobulin Antibody, Beta-2-microglobin Antibody

Beta 2-microglobulin is the low molecular weight component of HLA antigens and as such is involved in the presentation of endogenous antigens, activation of T lymphocytes and functional emergence of natural killer cells. It is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning and other conditions leading renal tubular dysfunction.

Immunohistochemistry

Immunohistochemistry
Anti-B2M antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2249 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-B2M antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2249 concentration 5 ug/ml.

Requested From: United States
Date Requested: 5/22/2015

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