Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
AXL Antibody, AXL transforming sequence/gene Antibody, Oncogene AXL Antibody, AXL oncogene Antibody, AXL receptor tyrosine kinase Antibody, JTK11 Antibody, Tyro7 Antibody, UFO Antibody
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL.
Phospho-Axl (Y779) in Human Stomach Cancer Tissue. Axl phosphorylated at site Y779 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Human Phospho-Axl (Y779) Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Tissue was stained using Anti-Rabbit HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells.
Phospho-Axl (Y779) in A172 Human Cell Line. Axl phosphorylated at Y779 (panels B, D) and total Axl (panels A, C) were assessed in immersion fixed A172 human glioblastoma cells incubated with (panels C, D ) or without ( panels A, B) pervanadate. PhosphoAxl was detected using Rabbit Anti-Human PhosphoAxl (Y779) Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red, panels B, D) and counterstained using DAPI (blue). Total Axl was detected using Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody. Cells were stained using NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green, panels A, C). Specific staining was localized to cytoplasm.
Detection of Human Phospho-Axl (Y779) by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with WI38 human lung cell line conditioned media (WI38 CM) for 15 minutes. PVDF membrane was probed with 1 ug/ml of Human Phospho-Axl (Y779) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for Phospho-Axl (Y779) at approximately 140 kD (as indicated). This experiment was conducted under reducing conditions.
Requested From: United States
Date Requested: 10/22/2016