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Catalog Number Size Price
LS-C60116-100 100 µg (1.28 mg/ml) $567 
ATR Antibody - Anti-ATR Antibody - Western Blot. Western blot of anti-ATR antibody shows detection of ATR in HeLa cell nuclear extract (lane 1). Lane 2 shows negligible staining after pre-incubation of antibody with the immunizing peptide (50 ug peptide for 1 h at room temperature followed by centrifugation). A 4-8% gradient gel was used for separation. Goat serum was used at 5% for blocking. The arrowhead corresponds to 301 kD ATR when compared to MW markers (Lane M). The primary antibody was used at a 1:1400 dilution.

Polyclonal Rabbit anti‑Human ATR Antibody (WB) LS‑C60116

Polyclonal Rabbit anti‑Human ATR Antibody (WB) LS‑C60116

Antibody:
ATR Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Xenopus, Fish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C60116-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ATR Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Xenopus, Fish
Format:
Unconjugated, Unmodified

Specifications

Description
ATR antibody LS-C60116 is an unconjugated rabbit polyclonal antibody to ATR from human. It is reactive with human, mouse, rat and other species. Validated for ELISA and WB.
Target
Human ATR
Synonyms
ATR | FRAP-related protein-1 | FCTCS | SCKL1 | MEC1 | Protein kinase ATR | Rad3 related protein | SCKL | FRAP-related protein 1 | FRP1 | XATR | Serine/threonine-protein kinase ATR | Ataxia telangiectasia and Rad3-related protein | mitosis entry checkpoint 1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Dog, Xenopus, Fish (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Dog, Xenopus, Fish (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATR protein.
Specificity
This antibody is specific for human ATR protein. A BLAST analysis was used to suggest cross-reactivity with ATR from human, mouse, rat, monkey, dog, fish and Xenopus sources based on a 100% homology with the immunizing sequence. Cross-reactivity with ATR from other sources has not been determined.
Applications
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:15000 - 1:70000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 301 kD in size corresponding to ATR by western blotting in the appropriate cell lysate or extract. Lysates from HeLa, U2OS, RAW264.7 or HEK293 cells are suggested for western blotting. ATR is ubiquitously expressed, with highest expression levels in the testes. Note that isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATR
Q13535 NM_001184 NP_001175.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024