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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ATP6V0D2 Antibody (aa251‑300) LS‑C111657
ATP6V0D2 antibody LS-C111657 is an unconjugated rabbit polyclonal antibody to ATP6V0D2 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C111657-50
50 µg
Unavailable

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ATP6V0D2 antibody LS-C102655 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Product Description

ATP6V0D2 antibody LS-C111657 is an unconjugated rabbit polyclonal antibody to ATP6V0D2 from human, mouse, rat and other species. Validated for WB.
About ATP6V0D2
Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis. Q8N8Y2 Q8N8Y2

ATP6V0D2 Antibody, ATP6D2 Antibody, V-ATPase subunit d 2 Antibody, VMA6 Antibody


Specifications

Target
Human ATP6V0D2
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
ATP6V0D2 antibody was raised against synthetic peptide located between aa251-300 of human ATP6V0D2 (Q8N8Y2, NP_689778). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Rabbit, Pig (100%); Monkey, Galago, Horse (92%); Bat, Guinea pig, Platypus (85%); Elephant (84%); Opossum (83%).
Blocking Peptide
LS-E20059 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111657. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa251-300
Specificity
Human ATP6V0D2
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

ATP6V0D2 antibody LS-C111657 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATP6V0D2 antibody LS-C111657 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATP6V0D2 antibody LS-C111657 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATP6V0D2 antibody LS-C111657 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATP6V0D2 antibody LS-C111657 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATP6V0D2 antibody LS-C111657 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATP6V0D2 antibody LS-C111657 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATP6V0D2 antibody LS-C111657 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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