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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ATP6AP1 Antibody (clone 3A2) LS‑C196724
ATP6AP1 antibody LS-C196724 is an unconjugated mouse monoclonal antibody to ATP6AP1 from human and rat. Validated for ELISA and WB.
Catalog
Size
Price
LS-C196724-100
100 µg
Unavailable

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Product Description

ATP6AP1 antibody LS-C196724 is an unconjugated mouse monoclonal antibody to ATP6AP1 from human and rat. Validated for ELISA and WB.
About ATP6AP1
ATP6AP1 is a component of a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. NM_001183 NP_001174.2

ATP6AP1 Antibody, 16A Antibody, ATP6IP1 Antibody, ATP6S1 Antibody, Ac45 Antibody, H-ATPase subunit Antibody, ORF Antibody, V-ATPase subunit S1 Antibody, VATPS1 Antibody, XAP-3 Antibody, Protein XAP-3 Antibody, V-ATPase Ac45 subunit Antibody, V-ATPase S1 subunit Antibody, CF2 Antibody, V-ATPase S1 accessory protein Antibody, XAP3 Antibody


Specifications

Target
Human ATP6AP1
Host
Mouse
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [3A2]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
ATP6AP1 antibody was raised against aTP6AP1 (NP_001174, aa51-150 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human ATP6AP1
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot analysis of ATP6AP1 expression in transfected 293T cell line by ATP6AP1 monoclonal antibody (M01), clone 3A2.Lane 1: ATP6AP1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ATP6AP1 expression in transfected 293T cell line by ATP6AP1 monoclonal antibody (M01), clone 3A2.Lane 1: ATP6AP1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.

Western blot

ATP6AP1 monoclonal antibody (M01), clone 3A2. Western Blot analysis of ATP6AP1 expression in PC-12.
ATP6AP1 monoclonal antibody (M01), clone 3A2. Western Blot analysis of ATP6AP1 expression in PC-12.

Western blot

ATP6AP1 monoclonal antibody (M01), clone 3A2 Western Blot analysis of ATP6AP1 expression in HeLa.
ATP6AP1 monoclonal antibody (M01), clone 3A2 Western Blot analysis of ATP6AP1 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ATP6AP1 is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ATP6AP1 is 0.1 ng/ml as a capture antibody.

Western blot

Western Blot analysis of ATP6AP1 expression in transfected 293T cell line by ATP6AP1 monoclonal antibody (M01), clone 3A2.Lane 1: ATP6AP1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ATP6AP1 expression in transfected 293T cell line by ATP6AP1 monoclonal antibody (M01), clone 3A2.Lane 1: ATP6AP1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.

Western blot

ATP6AP1 monoclonal antibody (M01), clone 3A2. Western Blot analysis of ATP6AP1 expression in PC-12.
ATP6AP1 monoclonal antibody (M01), clone 3A2. Western Blot analysis of ATP6AP1 expression in PC-12.

Western blot

ATP6AP1 monoclonal antibody (M01), clone 3A2 Western Blot analysis of ATP6AP1 expression in HeLa.
ATP6AP1 monoclonal antibody (M01), clone 3A2 Western Blot analysis of ATP6AP1 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ATP6AP1 is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ATP6AP1 is 0.1 ng/ml as a capture antibody.

Western blot

Western Blot analysis of ATP6AP1 expression in transfected 293T cell line by ATP6AP1 monoclonal antibody (M01), clone 3A2.Lane 1: ATP6AP1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ATP6AP1 expression in transfected 293T cell line by ATP6AP1 monoclonal antibody (M01), clone 3A2.Lane 1: ATP6AP1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.

Western blot

ATP6AP1 monoclonal antibody (M01), clone 3A2. Western Blot analysis of ATP6AP1 expression in PC-12.
ATP6AP1 monoclonal antibody (M01), clone 3A2. Western Blot analysis of ATP6AP1 expression in PC-12.

Western blot

ATP6AP1 monoclonal antibody (M01), clone 3A2 Western Blot analysis of ATP6AP1 expression in HeLa.
ATP6AP1 monoclonal antibody (M01), clone 3A2 Western Blot analysis of ATP6AP1 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ATP6AP1 is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ATP6AP1 is 0.1 ng/ml as a capture antibody.

Western blot

Western Blot analysis of ATP6AP1 expression in transfected 293T cell line by ATP6AP1 monoclonal antibody (M01), clone 3A2.Lane 1: ATP6AP1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ATP6AP1 expression in transfected 293T cell line by ATP6AP1 monoclonal antibody (M01), clone 3A2.Lane 1: ATP6AP1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.

Western blot

ATP6AP1 monoclonal antibody (M01), clone 3A2. Western Blot analysis of ATP6AP1 expression in PC-12.
ATP6AP1 monoclonal antibody (M01), clone 3A2. Western Blot analysis of ATP6AP1 expression in PC-12.

Western blot

ATP6AP1 monoclonal antibody (M01), clone 3A2 Western Blot analysis of ATP6AP1 expression in HeLa.
ATP6AP1 monoclonal antibody (M01), clone 3A2 Western Blot analysis of ATP6AP1 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ATP6AP1 is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ATP6AP1 is 0.1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/19/2018

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