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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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ATP5F1 Antibody (aa180‑229) LS‑C102297
ATP5F1 antibody LS-C102297 is an unconjugated rabbit polyclonal antibody to ATP5F1 from human, mouse, bovine and other species. Validated for WB.
Catalog
Size
Price
LS-C102297-50
50 µg
Unavailable

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Product Description

ATP5F1 antibody LS-C102297 is an unconjugated rabbit polyclonal antibody to ATP5F1 from human, mouse, bovine and other species. Validated for WB.
About ATP5F1
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. P24539 NM_001688 NP_001679.2

ATP5F1 Antibody, ATPase subunit b Antibody, H+-ATP synthase subunit b Antibody, PIG47 Antibody


Specifications

Target
Human ATP5F1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Rat, Horse, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
ATP5F1 antibody was raised against synthetic peptide located between aa180-229 of human ATP5F1 (P24539, NP_001679). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Elephant, Panda, Dog, Bovine, Rabbit, Guinea pig (100%); Orangutan, Rat, Horse, Pig (92%); Monkey, Opossum, Platypus (85%).
Blocking Peptide
LS-E17435 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102297. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa180-229
Specificity
Human ATP5F1
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

ATP5F1 antibody LS-C102297 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATP5F1 antibody LS-C102297 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATP5F1 antibody LS-C102297 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATP5F1 antibody LS-C102297 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATP5F1 antibody LS-C102297 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATP5F1 antibody LS-C102297 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATP5F1 antibody LS-C102297 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATP5F1 antibody LS-C102297 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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