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Anti-ATP4B Antibody (C-Terminus) LS-C135161

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

Most Popular ATP4B Antibodies

Anti-ATP4B Antibody (C-Terminus) LS-C137092
Rabbit Polyclonal (IgG) to Human ATP4B
Human
IHC, Western blot, ELISA
Unconjugated
Western blot Image
Anti-ATP4B Antibody (aa52-78) LS-C156651
Rabbit Polyclonal to Human ATP4B
Human, Mouse
Western blot, Flow Cytometry
Unconjugated
Western blot Image
Anti-ATP4B Antibody (clone 1D10) LS-C196717
Mouse Monoclonal [clone 1D10] (IgG2a,k) to Human ATP4B
Human
Western blot, ELISA
Unconjugated
Enzyme-Linked Immunosorbent Assay Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Rat ATP4B
Rat, Human, Mouse
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Rat ATP4B
Rabbit
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
Monkey, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

ATP4B antibody was raised against synthetic peptide from C-Terminus of rat Atp4b (P18598, NP_036642). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat (100%); Monkey, Galago, Marmoset, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch (92%); Turkey, Chicken, Xenopus (85%).
LS-E16283 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135161. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
C-Terminus
Rat ATP4B
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About ATP4B

P51164 NM_000705 NP_000696.1

ATP4B Antibody, ATP6B Antibody, Proton pump beta chain Antibody

ATP4B belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.

Western blot

Western blot
ATP4B antibody LS-C135161 Western blot of Rat Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: 
Date Requested: 3/28/2017

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