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| Catalog Number | Size | Price |
|---|---|---|
| LS-B491-0.05 | 0.05 ml (2.5 mg/ml) | $460 |
IHC‑plus™ Polyclonal Rabbit anti‑Human ATM Antibody (C‑Terminus, IHC, WB) LS‑B491
IHC‑plus™ Polyclonal Rabbit anti‑Human ATM Antibody (C‑Terminus, IHC, WB) LS‑B491
Note: This antibody replaces LS-C2761
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
ATM antibody LS-B491 is an unconjugated rabbit polyclonal antibody to human ATM (C-Terminus). Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human ATM
Synonyms
ATM | A-T mutated | ATA | ATC | ATD | ATE | AT mutated | Ataxia telangiectasia | ATDC | Serine-protein kinase ATM | AT1 | Ataxia telangiectasia mutated | TEL1 | TELO1
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A fragment of human ATM protein expressed in E. coli. The fragment relates to the C-Terminus (within the last third of the protein sequence)
Epitope
C-Terminus
Specificity
ATM.
Applications
- IHC
- IHC - Paraffin (10 - 20 µg/ml)
- Western blot (1:2500)
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B491 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B491 was determined to be 10-20 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ATM
Validation
Anti-ATM antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ATM antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ ATM Antibody (C-Terminus) for IHC, WB/Western, IP LS-B491 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Phospho-DeltaNp63 alpha -dependent microRNAs modulate chemoresistance of squamous cell carcinoma cells to cisplatin: at the crossroads of cell life and death. Ratovitski EA. FEBS letters. 2013 Aug;587:2536-41.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human, Monkey, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
Date Requested: 4/23/2024
