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Anti-ATG9A Antibody (aa717-746) LS-C156622

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ATG9A Antibodies

Anti-ATG9A Antibody (aa750-839) IHC-plus™ LS-B661
Rabbit Polyclonal to Human ATG9A
Human, Mouse, Rat, Bovine, Chicken, Primate
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ATG9A Antibody (C-Terminus) IHC-plus™ LS-B6984
Rabbit Polyclonal to Human ATG9A
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ATG9A Antibody (C-Terminus) LS-C187451
Rabbit Polyclonal (IgG) to Human ATG9A
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ATG9A
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human ATG9A
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)

Specificity and Use

ATG9A antibody was raised against kLH-conjugated synthetic peptide between amino acids 717-746 from C-terminal region of human ATG9A.
LS-E5622 - Lyophilized - 100 µg - $145.00
aa717-746
Human ATG9A

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About ATG9A

Q7Z3C6 NM_024085 NP_076990.4

ATG9A Antibody, APG9-like 1 Antibody, Autophagy 9-like 1 protein Antibody, Autophagy-related protein 9A Antibody, MGD3208 Antibody, MATG9 Antibody, APG9 autophagy 9-like 1 Antibody, APG9L1 Antibody, Autophagy related 9A Antibody

Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence

Immunofluorescence
Fluorescent image of U251 cells stained with ATG9A antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG9A primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG9A immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Western blot

Western blot
Western blot of anti-Autophagy APG9L1 Antibody in A2058 and A375 cell line lysates (35 ug/lane). APG9L1(arrow) was detected using the purified antibody.
 

Requested From: United States
Date Requested: 12/10/2016

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