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Anti-ATG4D Antibody (aa42-71) LS-C156602

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ATG4D Antibodies

Anti-ATG4D Antibody (aa444-457) IHC-plus™ LS-B6488
Goat Polyclonal to Human ATG4D
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ATG4D Antibody (clone 222CT15.4.1, APC) LS-C221250
Mouse Monoclonal [clone 222CT15.4.1] (IgG1,k) to Human ATG4D
Human
ICC, Immunofluorescence, Western blot, ELISA
APC Conjugated
Anti-ATG4D Antibody (clone 222CT15.4.1, FITC) LS-C221253
Mouse Monoclonal [clone 222CT15.4.1] (IgG1,k) to Human ATG4D
Human
ICC, Immunofluorescence, Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ATG4D
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human ATG4D
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)

Specificity and Use

ATG4D antibody was raised against kLH-conjugated synthetic peptide between amino acids 42-71 from N-terminal region of human ATG4D.
LS-E995 - Lyophilized - 100 µg - $145.00
aa42-71
Human AUTL4 / ATG4D

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About ATG4D

Q86TL0 NM_032885 NP_116274.3

ATG4D Antibody, APG4-D Antibody, APG4D Antibody, AUTL4 Antibody, APG4 autophagy 4 homolog D Antibody, Cysteine protease ATG4D Antibody, Autophagin-4 Antibody

Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy.

Immunohistochemistry

Immunohistochemistry
ATG4D Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Immunofluorescence
Fluorescent image of U251 cells stained with ATG4D antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG4D primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). ATG4D immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Western blot

Western blot
ATG4D Antibody western blot of mouse liver tissue lysates (35 ug/lane). The ATG4D antibody detected the ATG4D protein (arrow).
 

Requested From: United States
Date Requested: 12/9/2016

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