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Catalog Number	Size	Price
LS-C156602-400	400 µl	$393

ATG4D Antibody - ATG4D Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.

ATG4D Antibody - Fluorescent image of U251 cells stained with ATG4D antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG4D primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). ATG4D immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

ATG4D Antibody - ATG4D Antibody western blot of mouse liver tissue lysates (35 ug/lane). The ATG4D antibody detected the ATG4D protein (arrow).

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Polyclonal Rabbit anti‑Human ATG4D Antibody (aa42‑71, IHC, IF, WB) LS‑C156602

Name: Anti-ATG4D Antibody | Rabbit anti-Human Polyclonal IF,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C156602-400
Price: 393.0000 USD
Availability: InStock

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Antibody:

ATG4D Rabbit anti-Human Polyclonal (aa42-71) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$393

LS-C156602-400

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ATG4D Rabbit anti-Human Polyclonal (aa42-71) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

ATG4D antibody LS-C156602 is an unconjugated rabbit polyclonal antibody to ATG4D (aa42-71) from human. It is reactive with human and mouse. Validated for IF, IHC and WB.

Target

Human ATG4D

Synonyms

See All ATG4D Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Ammonium sulfate precipitation

Modifications

Unmodified

Epitope

aa42-71

Specificity

This ATG4D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-71 amino acids from the N-terminal region of human ATG4D.

Applications

IHC
IHC - Paraffin (1:50 - 1:100)
Immunofluorescence (1:200)
Western blot (1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About ATG4D

Uniprot: Q86TL0 NCBI: NM_032885 NP_116274.3