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Anti-ATG3 Antibody (clone 4F6) LS-C173219

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.93 mg/ml $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ATG3 Antibodies

Anti-ATG3 Antibody (Internal) LS-C115848
Rabbit Polyclonal to Human ATG3
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated
Immunofluorescence Image
Anti-ATG3 Antibody (aa1-314) IHC-plus™ LS-B13789
Rabbit Polyclonal (IgG) to Human ATG3
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 4F6] (IgG1) to Human ATG3
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human ATG3
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [4F6]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (1:150)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

ATG3 antibody was raised against full length human recombinant protein of human ATG3(NP_071933) produced in HEK293T cell.
Human ATG3

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About ATG3

Q9NT62 NM_022488 NP_071933.2

ATG3 Antibody, APG3-LIKE Antibody, APG3 Antibody, APG3L Antibody, Autophagy related 3 Antibody, HApg3 Antibody, PC3-96 Antibody, Protein PC3-96 Antibody, Autophagy-related protein 3 Antibody

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATG3 mouse monoclonal antibody.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Human pancreas tissue using anti-ATG3 mouse monoclonal antibody.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Human endometrium tissue using anti-ATG3 mouse monoclonal antibody.

Immunofluorescence

Immunofluorescence
Anti-ATG3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATG3.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATG3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATG3.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATG3 antibody, and then analyzed by flow cytometry.

Requested From: United States
Date Requested: 12/10/2016

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