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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ATF5 Antibody (aa188‑216) LS‑C156564
ATF5 antibody LS-C156564 is an unconjugated rabbit polyclonal antibody to human ATF5. Validated for WB.
Catalog
Size
Price
LS-C156564-400
400 µl
Unavailable

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ATF5 antibody LS-C112137 Western blot of Fetal Liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Immunohistochemical analysis of ATF5 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

ATF5 antibody LS-C156564 is an unconjugated rabbit polyclonal antibody to human ATF5. Validated for WB.
About ATF5
Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Q9Y2D1 NM_012068 NP_036200.2

ATF5 Antibody, HMFN0395 Antibody, Transcription factor ATFx Antibody, ATFX Antibody


Specifications

Target
Human ATF5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
Blocking Peptide
LS-E3773 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C156564. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa188-216
Specificity
This ATF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-216 amino acids from the C-terminal region of human ATF5.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

ATF5 Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The ATF5 antibody detected the ATF5 protein (arrow).
ATF5 Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The ATF5 antibody detected the ATF5 protein (arrow).

Western blot

ATF5 Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The ATF5 antibody detected the ATF5 protein (arrow).
ATF5 Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The ATF5 antibody detected the ATF5 protein (arrow).

Western blot

ATF5 Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The ATF5 antibody detected the ATF5 protein (arrow).
ATF5 Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The ATF5 antibody detected the ATF5 protein (arrow).

Western blot

ATF5 Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The ATF5 antibody detected the ATF5 protein (arrow).
ATF5 Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The ATF5 antibody detected the ATF5 protein (arrow).


Requested From: 
Date Requested: 6/24/2018

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