Human, Dog, Pig
(tested or 100% immunogen sequence identity)
Mouse, Rat (at least 90% immunogen sequence identity)
Also available conjugated with Biotin
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
ATF4 antibody was raised against synthetic peptide located between aa182-231 of human ATF4 (P18848, NP_001666). Percent identity by BLAST analysis: Human (100%); Mouse, Rat (90%); Pig (81%).
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Lyophilized from PBS, 2% sucrose.
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.