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Anti-ATF1 Antibody (N-Terminus) IHC-plus™ LS-B1511

Note: This antibody replaces LS-C30199
Catalog Size Price
LS-B1511-50 50 µg Unavailable

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66 ATF1 Antibodies

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Anti-ATF1 Antibody (aa179-228) IHC-plus™ LS-B6733
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Anti-ATF1 Antibody (C-Terminus) LS-C351857
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100% Guaranteed
Rabbit Polyclonal (IgG) to Human ATF1
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human ATF1
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

ATF1 antibody was raised against synthetic peptide from N-Terminus of human ATF1 (P18846, NP_005162). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Guinea pig (100%); Opossum (91%); Lizard, Xenopus (85%).
LS-E13878 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1511. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
N-Terminus
Human ATF1
Immunohistochemistry: LS-B1511 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1511 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About ATF1

P18846 NM_005171 NP_005162.1

ATF1 Antibody, ATF-1 Antibody, EWS-ATF1 Antibody, FUS/ATF-1 Antibody, Protein TREB36 Antibody, TREB36 Antibody

ATF1 is an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities.

Immunohistochemistry

Immunohistochemistry
Anti-ATF1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1511 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
ATF1 antibody LS-B1511 Western blot of Fetal thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Fetal Thymus Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 12/10/2017

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