LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
ATAD5 / ELG1 Antibody (aa1537‑1548) LS‑C54550
ELG1 antibody LS-C54550 is an unconjugated goat polyclonal antibody to mouse ELG1 (ATAD5). Validated for Peptide-ELISA.
Antibody
Catalog
Size
Price
LS-C54550-100
100 µg
$335

Popular ATAD5 / ELG1 Products

Antibody
Species: Human
Applications: Western blot, ELISA
Anti-Elg1 Antibody - Western Blot. Western blot of Affinity Purified anti-Elg1 antibody shows detection of a band ~120 kD corresponding to human Elg1 (arrowhead) in various cell lysates. Lanes contain ~5 ug of HeLa nuclear extract (1), HeLa (2), A431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500. The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2 m exposure time. The expected molecular weight of Elg1 is 120kD according to Kanellis P et al. 2003, although the predicted molecular weight is 207 kD. The 50kD bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 kD and 50 kD bands are not observed when antibody is pre-incubated with peptide (data not shown).
Species: Human
Applications: Western blot, ELISA
Detection of Human ATAD5/FRAG1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa and 293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-ATAD5/FRAG1 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Species: Human
Applications: Western blot, Immunoprecipitation, Proximity Ligation Assay
Western blot analysis of A431 cell, using ATAD5 Polyclonal Antibody at dilution of 1:400.
Species: Human
Applications: Western blot, ELISA
Western blot analysis of A431 cell, using ATAD5 Polyclonal Antibody at dilution of 1:750.
Species: Human
Applications: Western blot, ELISA

Product Description

ELG1 antibody LS-C54550 is an unconjugated goat polyclonal antibody to mouse ELG1 (ATAD5). Validated for Peptide-ELISA.
About ATAD5 / ELG1
Involved in DNA damage response. Involved in a RAD9A-related damage checkpoint, a pathway that is important in determining whether DNA damage is compatible with cell survival or whether it requires cell elimination by apoptosis. Modulates the RAD9A interaction with BCL2 and thereby induces DNA damages-induced apoptosis. Q96QE3 NM_024857 NP_079133.3

ATAD5 Antibody, C17orf41 Antibody, FRAG1 Antibody, ELG1 Antibody


Specifications

Target
Mouse ATAD5 / ELG1
Host
Goat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Failed Applications
  • IHC - Paraffin
Immunogen
ATAD5 / ELG1 antibody was raised against synthetic peptide C-QSKDQPLRKSQK from an internal region of mouse ATAD5 (NP_001025027.1). Percent identity with other species by BLAST analysis: Mouse (100%), Hamster (83%).
Blocking Peptide
LS-E26272 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C54550. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1537-1548
Specificity
Mouse ATAD5.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

Publications (0)


Customer Reviews (0)


Requested From: United States
Date Requested: 9/18/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy