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Anti-Aspartate Aminotransferase Antibody IHC-plus™ LS-B24

Note: This antibody replaces LS-C18751
Catalog Size Price
LS-B24-50 50 µl (10 mg/ml) Unavailable

Most Popular Aspartate Aminotransferase Antibodies

Anti-Aspartate Aminotransferase Antibody IHC-plus™ LS-B24
Sheep Polyclonal to Pig Aspartate Aminotransferase
Pig, Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Anti-GOT2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B24 concentration 20 ug/ml.
Anti-Aspartate Aminotransferase Antibody (aa22-35) LS-C54928
Goat Polyclonal to Human Aspartate Aminotransferase
Human
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Antibody (0.5 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-Aspartate Aminotransferase Antibody (N-Terminus) LS-C81182
Rabbit Polyclonal (IgG) to Human Aspartate Aminotransferase
Human, Monkey, Mouse, Rat, Bat, Bovine, Goat, Guinea pig, Horse, Pig, Rabbit, Zebrafish
Western blot
Unconjugated
HepG2 cell lysate. Antibody concentration: 5.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Aspartate Aminotransferase Antibody (aa157-167) IHC-plus™ LS-B2405
Goat Polyclonal to Human Aspartate Aminotransferase
Human, Rat, Hamster
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-GOT1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2405 concentration 5 ug/ml.
Anti-Aspartate Aminotransferase Antibody (N-Terminus) LS-C107785
Rabbit Polyclonal (IgG) to Human Aspartate Aminotransferase
Human, Monkey, Mouse, Rat, Bat, Bovine, Goat, Guinea pig, Horse, Pig, Rabbit, Zebrafish
Western blot
Unconjugated
Aspartate Aminotransferase antibody Western Blot of NCI-H226 . Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

100% Guaranteed Publications (2)
Sheep Polyclonal to Pig Aspartate Aminotransferase
Pig, Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated

Details

Pig Aspartate Aminotransferase
Sheep
Pig, Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Purified IgG
Unmodified

Applications

  • IHC
  • IHC - Paraffin (20 µg/ml)
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation

Specificity and Use

Aspartate transaminase purified from pig heart.
Aspartate Transaminase
Immunohistochemistry: LS-B24 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B24 was determined to be 20 ug/ml.

Packaging

0.015 M potassium phosphate buffer, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide.
Short term: -20°C; Long term: -20°C.
For research use only.

Publications (2)

1
Stimulation of angiogenesis, neurogenesis and regeneration by side population cells from dental pulp. Ishizaka R, Hayashi Y, Iohara K, Sugiyama M, Murakami M, Yamamoto T, Fukuta O, Nakashima M. Biomaterials. 2013 Mar;34:1888-97. [Full Text Article] [PubMed:23245334]
2
CXCL14 and MCP1 are potent trophic factors associated with cell migration and angiogenesis leading to higher regenerative potential of dental pulp side population cells. Hayashi Y, Murakami M, Kawamura R, Ishizaka R, Fukuta O, Nakashima M. Stem cell research & therapy. 2015 6:111. (WB; Pig) [Full Text Article] [PubMed:26021377] [PMC:PMC4488059]

About Aspartate Aminotransferase

P17174 NM_002079 NP_002070.1

GOT1 Antibody, Aspartate Aminotransferase Antibody, ASTQTL1 Antibody, Growth-inhibiting protein 18 Antibody, Transaminase A Antibody, GIG18 Antibody

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide.

Immunohistochemistry

Anti-GOT2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B24 concentration 20 ug/ml.
Anti-GOT2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B24 concentration 20 ug/ml.

Requested From: 
Date Requested: 5/23/2018

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