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Anti-ASCL1 / MASH1 Antibody LS-C69310

Ordering

Wt. Vol. Conc. Price
100 µg 200 µl 0.5 mg/ml $485
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ASCL1 / MASH1 Antibodies

Anti-ASCL1 / MASH1 Antibody LS-C69310
Rat Monoclonal (IgG2a) to Mouse ASCL1 / MASH1
Mouse
ICC, Western blot, ELISA
Unconjugated
Anti-ASCL1 / MASH1 Antibody (Glu158) LS-C177728
Rabbit Polyclonal to Human ASCL1 / MASH1
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal (IgG2a) to Mouse ASCL1 / MASH1
Mouse
ICC, Western blot, ELISA
Unconjugated

Details

Mouse ASCL1 / MASH1
Rat
Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • ICC
  • Western blot (1 - 2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

ASCL1 / MASH1 antibody was raised against e. coli-derived recombinant mouse rmMash1.
Recognizes mouse Mash1. Species sequence homology: human 97%.
Suitable for use in Immunocytochemistry, Western blot, and Direct ELISA. Western Blot: 1-2 ug/ml. Detection limit is ~1 ng/lane under non-reducing and. reducing conditions. Chemiluminescent detection with WesternGlo Chemiluminescent. Detection Substrate will increase sensitivity by 5 to 50 fold. Immunocytochemistry:-15 ug/ml will detect Mash1 on retinoic acid-differentiated human NTERA-2 cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1%. BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4C followed by Rhodamine Red-coupled anti-rat IgG at RT in the dark for one hour. Wash cells with PBS containing 0.1% BSA. Direct ELISA: 0.5-1.0 ug/ml The detection limit 1 is 0.5 ng/well.

Packaging

Lyophilized from PBS, 5% trehalose.
200 µl Sterile PBS
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid frequent freeze thaws.
For research use only.

About ASCL1 / MASH1

P50553 NM_004316 NP_004307.2

ASCL1 Antibody, Achaete-scute homolog 1 Antibody, ASH-1 Antibody, BHLHa46 Antibody, Achaete scute protein Antibody, Achaete-scute complex-like 1 Antibody, HASH1 Antibody, MASH1 Antibody, ASH1 Antibody

ACSL1 is a member of the basic helix-loop-helix (BHLH) family of transcription factors. It activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. ACSL1 plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. The protein is highly expressed in medullary thyroid cancer and small cell lung cancer and may be a useful marker for these cancers.

Requested From: United States
Date Requested: 12/9/2016

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