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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ASCC1 Antibody (C‑Terminus) LS‑C135408
ASCC1 antibody LS-C135408 is an unconjugated rabbit polyclonal antibody to ASCC1 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C135408-50
50 µg
Unavailable

Popular ASCC1 Products

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ASCC1 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASCC1.
Species: Human
Applications: Western blot, Flow Cytometry
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASCC1.
Species: Human
Applications: Western blot, Flow Cytometry
Anti-ASCC1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ASCC1.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
Immunohistochemistry of paraffin-embedded human tonsil using antibody at dilution of 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA

Product Description

ASCC1 antibody LS-C135408 is an unconjugated rabbit polyclonal antibody to ASCC1 from human, mouse, rat and other species. Validated for WB.
About ASCC1
ASCC1 is a subunit of the activating signal cointegrator 1 (ASC-1) complex. The ASC-1 complex is a transcriptional coactivator that plays an important role in gene transactivation by multiple transcription factors including activating protein 1 (AP-1), nuclear factor kappa-B (NF-kB) and serum response factor (SRF). The encoded protein contains an N-terminal KH-type RNA-binding motif which is required for AP-1 transactivation by the ASC-1 complex. Q8N9N2 NM_015947 NP_057031.2

ASCC1 Antibody, ASC1p50 Antibody, ASC-1 complex subunit p50 Antibody, p50 Antibody, CGI-18 Antibody, Em:AC022392.3 Antibody, Trip4 complex subunit p50 Antibody


Specifications

Target
Rat ASCC1
Host
Rabbit
Reactivity
Rat, Human, Mouse, Bovine, Dog, Goat, Horse, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
ASCC1 antibody was raised against synthetic peptide from C-Terminus of rat Ascc1 (NP_001007633). Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Goat, Dog, Bovine, Rabbit, Horse (100%); Monkey, Galago, Elephant, Panda, Bat (92%).
Blocking Peptide
LS-E20668 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135408. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Rat ASCC1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

ASCC1 antibody LS-C135408 Western blot of Rat liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ASCC1 antibody LS-C135408 Western blot of Rat liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ASCC1 antibody LS-C135408 Western blot of Rat liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ASCC1 antibody LS-C135408 Western blot of Rat liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ASCC1 antibody LS-C135408 Western blot of Rat liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ASCC1 antibody LS-C135408 Western blot of Rat liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ASCC1 antibody LS-C135408 Western blot of Rat liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ASCC1 antibody LS-C135408 Western blot of Rat liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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