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Anti-ARSB / Arylsulfatase B Antibody (aa359-372) IHC-plus™ LS-B2300

Note: This antibody replaces LS-C54544

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml Unavailable

Most Popular ARSB / Arylsulfatase B Antibodies

Anti-ARSB / Arylsulfatase B Antibody (aa359-372) IHC-plus™ LS-B2300
Goat Polyclonal to Human ARSB / Arylsulfatase B
Human, Monkey
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ARSB / Arylsulfatase B Antibody (aa464-493) LS-C100667
Rabbit Polyclonal to Human ARSB / Arylsulfatase B
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ARSB / Arylsulfatase B Antibody (clone 2G6) LS-C139410
Mouse Monoclonal [clone 2G6] (IgG1,k) to Human ARSB / Arylsulfatase B
Human
Western blot, ELISA
Unconjugated
Enzyme-Linked Immunosorbent Assay Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human ARSB / Arylsulfatase B
Human, Monkey
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human ARSB / Arylsulfatase B
Goat
Human, Monkey (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • ELISA (1:64000)

Specificity and Use

ARSB / Arylsulfatase B antibody was raised against synthetic peptide C-KLARGHTNGTKPLD from an internal region of human ARSB (NP_000037.2; NP_942002.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%); Elephant (93%); Monkey, Bovine, Bat, Cat, Panda, Horse, Chicken (86%).
LS-E26477 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2300. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa359-372
Human ARSB. This antibody is expected to recognise both reported isoforms (NP_000037.2; NP_942002.1).
Immunohistochemistry: LS-B2300 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2300 was determined to be 5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About ARSB / Arylsulfatase B

P15848 NM_000046 NP_000037.2

ARSB Antibody, Arylsulfatase B Antibody, ASB Antibody, G4S Antibody, MPS6 Antibody

Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Immunohistochemistry

Immunohistochemistry
Anti-ARSB antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2300 concentration 5 ug/ml.

Western blot

Western blot
Antibody (0.03 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 3/23/2017

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