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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ARID5B Antibody (aa77‑126) LS‑C201585
ARID5B antibody LS-C201585 is an unconjugated rabbit polyclonal antibody to ARID5B from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C201585-50
50 µg (1 mg/ml)
Unavailable

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Product Description

ARID5B antibody LS-C201585 is an unconjugated rabbit polyclonal antibody to ARID5B from human, mouse, rat and other species. Validated for WB.
About ARID5B
Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. Q14865 M73837 AAA59870.1

ARID5B Antibody, DESRT Antibody, Modulator recognition factor 2 Antibody, MRF2 Antibody, MRF-2 Antibody, MRF1-like protein Antibody


Specifications

Target
Human ARID5B
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
ARID5B antibody was raised against synthetic peptide located between aa77-126 of human ARID5B (Q14865-3, NP_115575). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Platypus (100%); Chicken (92%).
Epitope
aa77-126
Specificity
Human ARID5B
Presentation
Lyophilized from PBS, 2% sucrose.
Reconstitution
50 µl Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of Human HepG2. ARID5B antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human HepG2. ARID5B antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human HepG2. ARID5B antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human HepG2. ARID5B antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human HepG2. ARID5B antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human HepG2. ARID5B antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human HepG2. ARID5B antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human HepG2. ARID5B antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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