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Anti-ARHGEF25 / GEFT Antibody LS-C155646

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ARHGEF25 / GEFT Antibodies

Anti-ARHGEF25 / GEFT Antibody (aa506-555) LS-C82758
Rabbit Polyclonal (IgG) to Human ARHGEF25 / GEFT
Human, Rat
Western blot
Unconjugated
Western blot Image
Anti-ARHGEF25 / GEFT Antibody LS-C410581
Rabbit Polyclonal (IgG) to Human ARHGEF25 / GEFT
Human, Mouse, Rat
IHC, Western blot, Immunoprecipitation
Unconjugated
Anti-ARHGEF25 / GEFT Antibody (aa506-555, Biotin) LS-C457549
Rabbit Polyclonal (IgG) to Human ARHGEF25 / GEFT
Human, Chimpanzee, Orangutan, Gibbon, Rat
Western blot
Biotin Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human ARHGEF25 / GEFT
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human ARHGEF25 / GEFT
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

ARHGEF25 / GEFT antibody was raised against recombinant protein fragment contain a sequence corresponding to a region within amino acids 226 and 446 of GEFT. Percent identity by BLAST analysis: Human (100%); Mouse (86%).
Human ARHGEF25 / GEFT
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Packaging

0.1 M Tris-glycine, pH 7, 20% Glycerol, 0.01% Thimerosal
Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
For research use only.

About ARHGEF25 / GEFT

Q86VW2 AA463333 Q86VW2

ARHGEF25 Antibody, GEFT Antibody, RhoA/RAC/CDC42 exchange factor Antibody, p63RhoGEF Antibody, RAC/CDC42 exchange factor Antibody

May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Breast ca, using GEFT antibody at 1:250 dilution.

Western blot

Western blot
Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE ARHGEF25 / GEFT antibody diluted at 1:1000

Requested From: United States
Date Requested: 12/8/2016

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