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Anti-ARHGDIA / RHOGDI Antibody (clone 1F2) LS-C115130

Ordering

Wt. Vol. Conc. Price
- 100 µl - $350
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ARHGDIA / RHOGDI Antibodies

Anti-ARHGDIA / RHOGDI Antibody IHC-plus™ LS-B8714
Rabbit Polyclonal (IgG) to Human ARHGDIA / RHOGDI
Human, Mouse, Bovine
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1F2] (IgG1) to Human ARHGDIA / RHOGDI
Human
Western blot, Flow Cytometry
Unconjugated

Details

Human ARHGDIA / RHOGDI
Mouse
IgG1 Monoclonal [1F2]
Unconjugated
Affinity purified
Unmodified

Applications

  • Western blot (1:200 - 1:1000)
  • Flow Cytometry (1:100)

Specificity and Use

ARHGDIA / RHOGDI antibody was raised against full length human recombinant protein of human ARHGDIA (NP_004300) produced in HEK293T cell
Human ARHGDIA
Concentration: 0.53 mg/ml (Lot dependent)

Packaging

PBS (PH 7.3), 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About ARHGDIA / RHOGDI

P52565 NM_004309 NP_004300.1

ARHGDIA Antibody, GDIA1 Antibody, Rho GDI 1 Antibody, Rho-GDI alpha Antibody, RHOGDI-1 Antibody, RHOGDI Antibody

Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation.

Western blot

Western blot
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGDIA monoclonal antibody.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGDIA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGDIA.

Flow Cytometry

Flow Cytometry
Flow cytometric Analysis of Jurkat cells, using anti-ARHGDIA antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

Flow Cytometry
Flow cytometric Analysis of Hela cells, using anti-ARHGDIA antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARHGDIA antibody, and then analyzed by flow cytometry.
 

Requested From: United States
Date Requested: 12/9/2016

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