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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ARHGDIA / RHOGDI Antibody (aa10‑19) LS‑C139612
RHOGDI antibody LS-C139612 is an unconjugated goat polyclonal antibody to RHOGDI (ARHGDIA) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C139612-100
100 µg
Unavailable

Popular ARHGDIA / RHOGDI Products

Antibody
Species: Human, Mouse, Bovine
Applications: Western blot, Immunoprecipitation
IHC of paraffin-embedded prostate tissue using anti-ARHGDIA mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Antibody
Species: Human, Mouse, Rat
Applications: IHC, Peptide Enzyme-Linked Immunosorbent Assay
Formalin-fixed and paraffin-embedded human brain tissue reacted with ARHGDIA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Anti-ARHGDIA / RHOGDI antibody IHC staining of human small intestine, lymphocytes in Peyer's patch. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Mouse, Bovine
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation

Product Description

RHOGDI antibody LS-C139612 is an unconjugated goat polyclonal antibody to RHOGDI (ARHGDIA) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
About ARHGDIA / RHOGDI
Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. P52565 NM_004309 NP_004300.1

ARHGDIA Antibody, GDIA1 Antibody, Rho GDI 1 Antibody, Rho-GDI alpha Antibody, RHOGDI-1 Antibody, RHOGDI Antibody


Specifications

Target
Human ARHGDIA / RHOGDI
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Chicken (tested or 100% immunogen sequence identity)
Predicted
Bat, Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Immunogen
Synthetic peptide C-QLAQIAAENE from an internal region of human ARHGDIA / RhoGDI (NP_004300.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Lizard (100%); Bat, Pufferfish, Zebrafish (90%); Salmon, Stickleback, Drosophila, Mosquito, Nematode, Paramecium (80%).
Blocking Peptide
LS-E27434 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C139612. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa10-19
Specificity
Human ARHGDIA / RhoGDI.
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 28kD band observed in Mouse and Rat Lung lysates (calculated MW of 23.4kD according to Mouse NP_598557.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

ARHGDIA antibody (1 ug/ml) staining of Mouse Lung lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ARHGDIA antibody (1 ug/ml) staining of Mouse Lung lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

ARHGDIA antibody (1 ug/ml) staining of Mouse Lung lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ARHGDIA antibody (1 ug/ml) staining of Mouse Lung lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

ARHGDIA antibody (1 ug/ml) staining of Mouse Lung lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ARHGDIA antibody (1 ug/ml) staining of Mouse Lung lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

ARHGDIA antibody (1 ug/ml) staining of Mouse Lung lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ARHGDIA antibody (1 ug/ml) staining of Mouse Lung lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/23/2018

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