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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ARH / LDLRAP1 Antibody (clone 4G4‑D5) IHC‑plus™ LS‑B4523
Note: This antibody replaces LS-C105129
LDLRAP1 antibody LS-B4523 is an unconjugated mouse monoclonal antibody to human LDLRAP1 (ARH). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4523-50
50 µg
Unavailable

Popular ARH / LDLRAP1 Products

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing showing cytoplasmic and some nuclear staining. T
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Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
LDLRAP1 antibody LS-C111631 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: Western blot
IHC of paraffin-embedded Human Kidney tissue using anti-LDLRAP1 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Immunohistochemistry of paraffin-embedded rat kidney tissue.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot

Product Description

LDLRAP1 antibody LS-B4523 is an unconjugated mouse monoclonal antibody to human LDLRAP1 (ARH). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About ARH / LDLRAP1
Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. Q5SW96 NP_056442.2

LDLRAP1 Antibody, ARH Antibody, ARH1 Antibody, FHCB1 Antibody, FHCB2 Antibody, LDL receptor adaptor protein Antibody, ARH2 Antibody


Specifications

Target
Human ARH / LDLRAP1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [4G4-D5]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ARH / LDLRAP1 antibody was raised against lDLRAP1 (AAH29770, 1 a.a. ~ 264 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Usage
Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells.
Presentation
PBS, pH 7.4
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-LDLRAP1 antibody IHC of human small intestine, muscularis propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.
Anti-LDLRAP1 antibody IHC of human small intestine, muscularis propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.

Immunohistochemistry

Anti-LDLRAP1 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.
Anti-LDLRAP1 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.

Immunohistochemistry

Anti-LDLRAP1 antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.
Anti-LDLRAP1 antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to LDLRAP1 on HeLa cell (antibody concentration 10 ug/ml).
Immunofluorescence of monoclonal antibody to LDLRAP1 on HeLa cell (antibody concentration 10 ug/ml).

Western blot

LDLRAP1 monoclonal antibody, clone 4G4-D5 Western blot of LDLRAP1 expression in HeLa NE.
LDLRAP1 monoclonal antibody, clone 4G4-D5 Western blot of LDLRAP1 expression in HeLa NE.

Western blot

Western blot of LDLRAP1 expression in transfected 293T cell line by LDLRAP1 monoclonal antibody, clone 4G4-D5.
Western blot of LDLRAP1 expression in transfected 293T cell line by LDLRAP1 monoclonal antibody, clone 4G4-D5.

Immunohistochemistry

Anti-LDLRAP1 antibody IHC of human small intestine, muscularis propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.
Anti-LDLRAP1 antibody IHC of human small intestine, muscularis propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.

Immunohistochemistry

Anti-LDLRAP1 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.
Anti-LDLRAP1 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.

Immunohistochemistry

Anti-LDLRAP1 antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.
Anti-LDLRAP1 antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to LDLRAP1 on HeLa cell (antibody concentration 10 ug/ml).
Immunofluorescence of monoclonal antibody to LDLRAP1 on HeLa cell (antibody concentration 10 ug/ml).

Western blot

LDLRAP1 monoclonal antibody, clone 4G4-D5 Western blot of LDLRAP1 expression in HeLa NE.
LDLRAP1 monoclonal antibody, clone 4G4-D5 Western blot of LDLRAP1 expression in HeLa NE.

Western blot

Western blot of LDLRAP1 expression in transfected 293T cell line by LDLRAP1 monoclonal antibody, clone 4G4-D5.
Western blot of LDLRAP1 expression in transfected 293T cell line by LDLRAP1 monoclonal antibody, clone 4G4-D5.

Immunohistochemistry

Anti-LDLRAP1 antibody IHC of human small intestine, muscularis propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.
Anti-LDLRAP1 antibody IHC of human small intestine, muscularis propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.

Immunohistochemistry

Anti-LDLRAP1 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.
Anti-LDLRAP1 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.

Immunohistochemistry

Anti-LDLRAP1 antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.
Anti-LDLRAP1 antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to LDLRAP1 on HeLa cell (antibody concentration 10 ug/ml).
Immunofluorescence of monoclonal antibody to LDLRAP1 on HeLa cell (antibody concentration 10 ug/ml).

Western blot

LDLRAP1 monoclonal antibody, clone 4G4-D5 Western blot of LDLRAP1 expression in HeLa NE.
LDLRAP1 monoclonal antibody, clone 4G4-D5 Western blot of LDLRAP1 expression in HeLa NE.

Western blot

Western blot of LDLRAP1 expression in transfected 293T cell line by LDLRAP1 monoclonal antibody, clone 4G4-D5.
Western blot of LDLRAP1 expression in transfected 293T cell line by LDLRAP1 monoclonal antibody, clone 4G4-D5.

Immunohistochemistry

Anti-LDLRAP1 antibody IHC of human small intestine, muscularis propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.
Anti-LDLRAP1 antibody IHC of human small intestine, muscularis propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.

Immunohistochemistry

Anti-LDLRAP1 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.
Anti-LDLRAP1 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.

Immunohistochemistry

Anti-LDLRAP1 antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.
Anti-LDLRAP1 antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4523 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to LDLRAP1 on HeLa cell (antibody concentration 10 ug/ml).
Immunofluorescence of monoclonal antibody to LDLRAP1 on HeLa cell (antibody concentration 10 ug/ml).

Western blot

LDLRAP1 monoclonal antibody, clone 4G4-D5 Western blot of LDLRAP1 expression in HeLa NE.
LDLRAP1 monoclonal antibody, clone 4G4-D5 Western blot of LDLRAP1 expression in HeLa NE.

Western blot

Western blot of LDLRAP1 expression in transfected 293T cell line by LDLRAP1 monoclonal antibody, clone 4G4-D5.
Western blot of LDLRAP1 expression in transfected 293T cell line by LDLRAP1 monoclonal antibody, clone 4G4-D5.


Requested From: 
Date Requested: 6/18/2018

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