Arginine, dimethyl

Rabbit

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

KLH-conjugated, synthetic peptide containing four symmetrical dimethyl-arginine-glycine repeats (KR*GR*GR*GR*G in which (R*) denotes symmetric dimethylation of arginine).

Recognizes proteins that contain multiple symmetrically dimethylated arginines, including the Sm protein B, B' and D. Does not recognize nonmethylated peptides, peptides containing asymmetrically dimethylated arginines, or a peptide containing a single symmetric dimethyl-arginine. Species cross-reactivity: human and mouse.

• ICC (1:500)
• Western blot
• Immunoprecipitation

Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Detects bands at 30, 32 and 16kD, corresponding to p60, Sm B, B' and D, respectively. Immunoprecipitation: Reported by an independent laboratory to immunoprecipitate proteins containing symmetric dimethyl-arginine. Immunocytochemistry: 1:500. Reported by an independent laboratory to immunostain Cajal bodies in the nuclei of HeLa, WI-38 and NIH/3T3 cells fixed with paraformaldehyde and permeabilized with triton.

0.05% Sodium Azide, 30% Glycerol

For research use only. Intended for use by laboratory professionals.

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