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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ARF1 Antibody IHC‑plus™ LS‑B4817 Best Seller
Note: This antibody replaces LS-C109519
ARF1 antibody LS-B4817 is an unconjugated rabbit polyclonal antibody to ARF1 from human, mouse and zebrafish. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4817-100
100 µl (0.68 mg/ml)
Unavailable

Popular ARF1 Products

Antibody
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
IHC (P) using Arf1 Antibody
Species: Human, Chimpanzee, Monkey, Mouse, Bat, Dog, Guinea pig, Hamster, Horse, Pig, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemistry of paraffin-embedded human small intestine tissue at dilution of 1:100
Species: Human
Applications: IHC, Immunofluorescence, ELISA

Product Description

ARF1 antibody LS-B4817 is an unconjugated rabbit polyclonal antibody to ARF1 from human, mouse and zebrafish. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About ARF1
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. P84077 NM_001658 NP_001649.1

ARF1 Antibody, ADP-ribosylation factor 1 Antibody


Specifications

Target
Human ARF1
Host
Rabbit
Reactivity
Human, Mouse, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC (1:100 - 1:200)
  • Immunofluorescence (1:100 - 1:200)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ARF1 antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 3 and 172 of ARF1.
Specificity
Recognizes human ARF1.
Usage
Cross-reactivity with mouse and zebrafish has been confirmed in Western blot only at this time.
Presentation
0.1 M Tris-glycine, pH 7, 10% Glycerol, 0.01% Thimerosal
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-ARF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.
Anti-ARF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.

Immunohistochemistry

Anti-ARF1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.
Anti-ARF1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of methanol-fixed HeLa, using ARF1 antibody at 1:200 dilution.
Immunofluorescence of methanol-fixed HeLa, using ARF1 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431. B: H1299. 12% SDS PAGE. ARF1 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: A431. B: H1299. 12% SDS PAGE. ARF1 antibody diluted at 1:1000.

Immunohistochemistry

Anti-ARF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.
Anti-ARF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.

Immunohistochemistry

Anti-ARF1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.
Anti-ARF1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of methanol-fixed HeLa, using ARF1 antibody at 1:200 dilution.
Immunofluorescence of methanol-fixed HeLa, using ARF1 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431. B: H1299. 12% SDS PAGE. ARF1 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: A431. B: H1299. 12% SDS PAGE. ARF1 antibody diluted at 1:1000.

Immunohistochemistry

Anti-ARF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.
Anti-ARF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.

Immunohistochemistry

Anti-ARF1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.
Anti-ARF1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of methanol-fixed HeLa, using ARF1 antibody at 1:200 dilution.
Immunofluorescence of methanol-fixed HeLa, using ARF1 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431. B: H1299. 12% SDS PAGE. ARF1 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: A431. B: H1299. 12% SDS PAGE. ARF1 antibody diluted at 1:1000.

Immunohistochemistry

Anti-ARF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.
Anti-ARF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.

Immunohistochemistry

Anti-ARF1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.
Anti-ARF1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4817 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of methanol-fixed HeLa, using ARF1 antibody at 1:200 dilution.
Immunofluorescence of methanol-fixed HeLa, using ARF1 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431. B: H1299. 12% SDS PAGE. ARF1 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: A431. B: H1299. 12% SDS PAGE. ARF1 antibody diluted at 1:1000.


Requested From: 
Date Requested: 6/18/2018

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