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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ARAF / ARAF1 / A‑RAF Antibody (phospho‑Tyr302) LS‑C198640
A-RAF antibody LS-C198640 is an unconjugated rabbit polyclonal antibody to A-RAF (ARAF / ARAF1) from human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C198640-50
50 µl
Unavailable
LS-C198640-100
100 µl
Unavailable

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Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

A-RAF antibody LS-C198640 is an unconjugated rabbit polyclonal antibody to A-RAF (ARAF / ARAF1) from human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
About ARAF / ARAF1 / A-RAF
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. P10398 NM_001654 NP_001645.1

ARAF Antibody, A RAF Antibody, A-RAF Antibody, A-raf-1 Antibody, PKS Antibody, PKS2 Antibody, Proto-oncogene A-Raf Antibody, Proto-oncogene A-Raf-1 Antibody, Ras-binding protein DA-Raf Antibody, Oncogene ARAF1 Antibody, ARAF1 Antibody, Proto-oncogene Pks Antibody, RAFA1 Antibody


Specifications

Target
Human ARAF / ARAF1 / A-RAF
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide derived from human A-RAF around the phosphorylation site of Tyr302.
Epitope
pTyr302
Specificity
A-RAF (Phospho-Tyr302) Antibody detects endogenous levels of A-RAF only when phosphorylated at tyrosine302.
Presentation
PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.

Immunofluorescence

Immunofluorescence of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HeLa cells treated with PMA 125ng/ml 30', using A-RAF (Phospho-Tyr302) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HeLa cells treated with PMA 125ng/ml 30', using A-RAF (Phospho-Tyr302) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.

Immunofluorescence

Immunofluorescence of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HeLa cells treated with PMA 125ng/ml 30', using A-RAF (Phospho-Tyr302) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HeLa cells treated with PMA 125ng/ml 30', using A-RAF (Phospho-Tyr302) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.

Immunofluorescence

Immunofluorescence of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HeLa cells treated with PMA 125ng/ml 30', using A-RAF (Phospho-Tyr302) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HeLa cells treated with PMA 125ng/ml 30', using A-RAF (Phospho-Tyr302) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.

Immunofluorescence

Immunofluorescence of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HeLa cells treated with PMA 125ng/ml 30', using A-RAF (Phospho-Tyr302) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HeLa cells treated with PMA 125ng/ml 30', using A-RAF (Phospho-Tyr302) Antibody. The lane on the right was incubated with synthetic peptide.


Requested From: 
Date Requested: 6/18/2018

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