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Anti-ARAF / ARAF1 / A-RAF Antibody (aa371-554) LS-C185401

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.85 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular ARAF / ARAF1 / A-RAF Antibodies

Anti-ARAF / ARAF1 / A-RAF Antibody (aa276-325) IHC-plus™ LS-B8371
Rabbit Polyclonal (IgG) to Human ARAF / ARAF1 / A-RAF
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ARAF / ARAF1 / A-RAF Antibody (aa248-277, APC) LS-C251599
Rabbit Polyclonal (IgG) to Human ARAF / ARAF1 / A-RAF
Human
Western blot, ELISA
APC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human ARAF / ARAF1 / A-RAF
Human
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human ARAF / ARAF1 / A-RAF
Rabbit
Human (tested or 100% immunogen sequence identity)
Rat, Bovine, Pig (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

ARAF / ARAF1 / A-RAF antibody was raised against recombinant fragment corresponding to a region within amino acids 371 and 554 of A-RAF (SwissProt P10398). Percent identity by BLAST analysis: Human (100%); Pig, Rat, Bovine (99%); Zebrafish (85%).
aa371-554
Human ARAF / A RAF
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About ARAF / ARAF1 / A-RAF

P10398 NM_001654 NP_001645.1

ARAF Antibody, A RAF Antibody, A-RAF Antibody, A-raf-1 Antibody, PKS Antibody, PKS2 Antibody, Proto-oncogene A-Raf Antibody, Proto-oncogene A-Raf-1 Antibody, Ras-binding protein DA-Raf Antibody, Oncogene ARAF1 Antibody, ARAF1 Antibody, Proto-oncogene Pks Antibody, RAFA1 Antibody

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Cal27 xenograft using ARAF antibody at 1:500 dilution.

Immunocytochemistry

Immunocytochemistry
Immunofluorescence of paraformaldehyde-fixed A431 using ARAF antibody at 1:200 dilution.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: Hela. 7.5% SDS PAGE. ARAF / A RAF antibody diluted at 1:1000.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. ARAF / A RAF antibody diluted at 1:1000.

Requested From: United States
Date Requested: 12/4/2016

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