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Anti-AR / Androgen Receptor Antibody (phospho-Ser213, clone 156C135.2) LS-C498

Note: This antibody replaces LS-C69003, LS-C20617

Ordering

Wt. Vol. Conc. Price
100 µg 200 µl 0.5 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular AR / Androgen Receptor Antibodies

Anti-AR / Androgen Receptor Antibody (clone AR 441) LS-C87494
Mouse Monoclonal [clone AR 441] (IgG1) to Human AR / Androgen Receptor
Human, Dog
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Gel shift
Unconjugated
Immunohistochemistry Image
Anti-AR / Androgen Receptor Antibody (N-Terminus) IHC-plus™ LS-B3326
Rabbit Polyclonal to Human AR / Androgen Receptor
Human, Mouse, Rat
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 156C135.2] (IgG) to Human AR / Androgen Receptor
Human, Monkey
Western blot
Unconjugated

Details

Human AR / Androgen Receptor
Mouse
Human, Monkey (tested or 100% immunogen sequence identity)
Bat (at least 90% immunogen sequence identity)
IgG Monoclonal [156C135.2]
Unconjugated
Protein G purified
Unmodified

Applications

Western blot (1 - 4 µg/ml)

Specificity and Use

AR / Androgen Receptor antibody was raised against this antibody was developed against a synthetic peptide corresponding to amino acids 207-221 (GRAREAS*GAPTSSKD) of human androgen receptor, containing the serine 213 phosphorylation site: GenBank Accession No. A39248. Note: S* refers to phosphorylated serine in the peptide sequence. Percent identity by BLAST analysis: Human, Chimpanzee, Baboon, Monkey, Marmoset (100%); Gorilla, Bat, Panda (93%); Dog, Bovine, Cat, Rabbit, Horse, Pig, Guinea pig (87%); Mouse, Rat, Hamster (80%).
pSer213
In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kD band was observed. Please see Lin et al. 2003 for additional details. The serine phosphorylation site recognized by this has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.

Packaging

PBS, 0.2% gelatin, 0.05% sodium azide.
Long term: -70°C; Short term: +4°C
For research use only.

About AR / Androgen Receptor

P10275 NM_000044 NP_000035.2

AR Antibody, Androgen receptor Antibody, Dihydrotestosterone receptor Antibody, DHTR Antibody, AIS Antibody, KD Antibody, NR3C4 Antibody, HUMARA Antibody, SBMA Antibody, TFM Antibody, SMAX1 Antibody, HYSP1 Antibody

The androgen receptor (AR) is a NR3 Steroid Receptor located on the X chromosome. AR is a phosphoprotein and acts as a steroid hormone-activated transcription factor for androgen-responsive genes. Mutations in the AR gene are associated with androgen insensitivity syndrome (CAIS) or testicular feminization syndrome, Reifenstein syndrome, and Kennedy spinal and bulbar muscular atrophy. They lead to symptoms such as low virilization, reduced sperm production, testicular atrophy, and infertility.

Western blot

Western blot
LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A)left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h.
 

Requested From: United States
Date Requested: 12/6/2016

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