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Anti-Apolipoprotein A-V Antibody (clone 1G5G9) IHC-plus™ LS-B2261

Note: This antibody replaces LS-C2602, LS-C47130

Ordering

Wt. Vol. Conc. Price
- 100 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Apolipoprotein A-V Antibodies

Anti-Apolipoprotein A-V Antibody (aa21-210) LS-C294029
Rabbit Polyclonal (IgG) to Mouse Apolipoprotein A-V
Mouse
Western blot, ELISA
Unconjugated
Western blot Image
Anti-Apolipoprotein A-V Antibody (aa21-210, Biotin) LS-C297265
Rabbit Polyclonal (IgG) to Mouse Apolipoprotein A-V
Mouse
Western blot, ELISA
Biotin Conjugated
Western blot Image
Anti-Apolipoprotein A-V Antibody (clone 1H4) LS-C338367
Mouse Monoclonal [clone 1H4] (IgG1) to Human Apolipoprotein A-V
Human, Monkey, Mouse
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1G5G9] (IgG1,k) to Human Apolipoprotein A-V
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human Apolipoprotein A-V
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1G5G9]
Unconjugated
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA

Specificity and Use

Apolipoprotein A-V antibody was raised against ni-NTA purified full-length recombinant human APOA5 expressed in E. coli strain BL21 (DE3). Percent identity by BLAST analysis: Bovine (83%); Rat (79%); Mouse (78%).
Mouse Monoclonal to Apo A5 (1G5G9).
Immunohistochemistry: LS-B2261 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2261 was determined to be 5 ug/ml.

Packaging

Ascites.
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
For research use only.

About Apolipoprotein A-V

Q6Q788 NM_052968 NP_443200.2

APOA5 Antibody, Apo-AV Antibody, APOAV Antibody, Apolipoprotein A5 Antibody, Apo-A5 Antibody, Apolipoprotein A-V Antibody, RAP3 Antibody, ApoA-V Antibody

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity).

Immunohistochemistry

Immunohistochemistry
Anti-APOA5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2261 concentration 5 ug/ml.

Western blot

Western blot
Western blot of anti- human APOA5 monoclonal antibody against human serum total protein (lane 2). Lane 1 is a MW standard.

Western blot

Western blot
Detection of Apo A5 in HepG2 cell lysate .
 

Requested From: United States
Date Requested: 12/6/2016

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