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Anti-APOB / Apolipoprotein B Antibody IHC-plus™ LS-B2592

Note: This antibody replaces LS-C20727

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

Most Popular APOB / Apolipoprotein B Antibodies

Anti-APOB / Apolipoprotein B Antibody (HRP) LS-C20730
Goat Polyclonal (IgG) to Human APOB / Apolipoprotein B
Human
Western blot, ELISA
HRP Conjugated
Anti-APOB / Apolipoprotein B Antibody IHC-plus™ LS-B2592
Goat Polyclonal (IgG) to Human APOB / Apolipoprotein B
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-APOB / Apolipoprotein B Antibody (aa3984-3995) IHC-plus™ LS-B3022
Goat Polyclonal to Human APOB / Apolipoprotein B
Human, Monkey
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-APOB / Apolipoprotein B Antibody IHC-plus™ LS-B6180
Rabbit Polyclonal (IgG) to Mouse APOB / Apolipoprotein B
Mouse, Human
IHC - Paraffin, Western blot, Immunodiffusion
Unconjugated
Immunohistochemistry Image
Anti-APOB / Apolipoprotein B Antibody LS-C149851
Mouse Monoclonal (IgG2b) to Human APOB / Apolipoprotein B
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human APOB / Apolipoprotein B
Human
IHC - Paraffin, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Human APOB / Apolipoprotein B
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)

Specificity and Use

APOB / Apolipoprotein B antibody was raised against affinity purified Apolipoprotein-B (hLDL).
Recognizes Apo B (monospecific) by IEP.
Immunohistochemistry: LS-B2592 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2592 was determined to be 5 ug/ml.

Packaging

0.05 M Tris-HCl, 0.5 M sodium chloride, pH 7.5, 0.1% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About APOB / Apolipoprotein B

P04114 NM_000384 NP_000375.2

APOB Antibody, ApoB-100 Antibody, Apolipoprotein B-48 Antibody, ApoB-48 Antibody, Apolipoprotein B Antibody, FLDB Antibody, LDLCQ4 Antibody, Mutant Apo B 100 Antibody, Apo B-100 Antibody, Apolipoprotein B-100 Antibody, Apolipoprotein B48 Antibody

Apolipoprotein B (ApoB) is the main apolipoprotein of chylomicrons and low density lipoproteins (LDL). The protein occurs in the plasma in 2 main isoforms, apoB-48 and apoB-100. The first is synthesized exclusively by the gut, the second by the liver. The intestinal (B-48) and hepatic (B-100) forms of apoB are coded by a single gene and by a single mRNA transcript larger than 16 kb. The 2 proteins share a common amino terminal sequence.

Immunohistochemistry

Immunohistochemistry
Anti-Apolipoprotein B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2592 concentration 5 ug/ml.

Requested From: 
Date Requested: 4/23/2017

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