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Antibodies > APOA1 / Apolipoprotein A 1 > LS-C355408
 
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LS-C355408-1 1 mg $857 
APOA1 / Apolipoprotein A 1 Antibody - Immunohistochemistry analysis of Apolipoprotein A-1 showing staining in the cytoplasm of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Apolipoprotein A-1 Mouse Monoclonal Antibody. Proteins were transferred to a Nitrocellulose Membrane using the G2 Fast Blotter, and blocked with 5% milk in TBST for at least 1 hour at room temperature. Apo A-1 was detected at ~50kD using an Apolipoprotein A-1 monoclonal antibody at a dilution of 1:1000 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG Fc-specific secondary antibody at a dilution of 1:40,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Dura.
APOA1 / Apolipoprotein A 1 Antibody - Apolipoprotein A-1 Western Blot
APOA1 / Apolipoprotein A 1 Antibody - Sandwich ELISA of Apolipoprotein A-1 was performed by coating wells of a 96-well plate with 100ul of an Apo A-1 rabbit oligoclonal antibody at a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 150ul of StartingBlock T20 (TBS) Blocking Buffer for 30 minutes, and 80ul of recombinant human Apo A-1 standards ranging from 1.6-1000ng/ml (A) or 100ul of diluted normal human serum or diluted serum from a patient with dyslipidemia (B) were incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of an Apo A-1 mouse monoclonal antibody was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of a biotinylated rabbit anti-mouse IgG Superclonal secondary antibody was incubated for 1 hour, followed by Streptavidin-HRP for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.
APOA1 / Apolipoprotein A 1 Antibody - Sandwich ELISA of Apolipoprotein A-1 was performed by coating wells of a 96-well plate with 100ul of an Apo A-1 rabbit oligoclonal antibody diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 150ul of StartingBlock T20 (TBS) Blocking Buffer for 30 minutes, and 80ul of recombinant human Apo A-1 or recombinant mouse Apo A-1 was added to the plate at concentrations ranging from 1.6-1000ng/ml and incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of an Apo A-1 mouse monoclonal antibody was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of an HRP-conjugated rabbit anti-mouse IgG cross-adsorbed secondary antibody was incubated for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.
APOA1 / Apolipoprotein A 1 Antibody - Immunohistochemistry analysis of Apolipoprotein A-1 showing staining in the cytoplasm of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Apolipoprotein A-1 Mouse Monoclonal Antibody. Proteins were transferred to a Nitrocellulose Membrane using the G2 Fast Blotter, and blocked with 5% milk in TBST for at least 1 hour at room temperature. Apo A-1 was detected at ~50kD using an Apolipoprotein A-1 monoclonal antibody at a dilution of 1:1000 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG Fc-specific secondary antibody at a dilution of 1:40,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Dura.
APOA1 / Apolipoprotein A 1 Antibody - Apolipoprotein A-1 Western Blot
APOA1 / Apolipoprotein A 1 Antibody - Sandwich ELISA of Apolipoprotein A-1 was performed by coating wells of a 96-well plate with 100ul of an Apo A-1 rabbit oligoclonal antibody at a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 150ul of StartingBlock T20 (TBS) Blocking Buffer for 30 minutes, and 80ul of recombinant human Apo A-1 standards ranging from 1.6-1000ng/ml (A) or 100ul of diluted normal human serum or diluted serum from a patient with dyslipidemia (B) were incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of an Apo A-1 mouse monoclonal antibody was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of a biotinylated rabbit anti-mouse IgG Superclonal secondary antibody was incubated for 1 hour, followed by Streptavidin-HRP for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.
APOA1 / Apolipoprotein A 1 Antibody - Sandwich ELISA of Apolipoprotein A-1 was performed by coating wells of a 96-well plate with 100ul of an Apo A-1 rabbit oligoclonal antibody diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 150ul of StartingBlock T20 (TBS) Blocking Buffer for 30 minutes, and 80ul of recombinant human Apo A-1 or recombinant mouse Apo A-1 was added to the plate at concentrations ranging from 1.6-1000ng/ml and incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of an Apo A-1 mouse monoclonal antibody was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of an HRP-conjugated rabbit anti-mouse IgG cross-adsorbed secondary antibody was incubated for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.
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Monoclonal Mouse anti‑Human APOA1 / Apolipoprotein A 1 Antibody (clone 6001, IHC, WB) LS‑C355408

Monoclonal Mouse anti‑Human APOA1 / Apolipoprotein A 1 Antibody (clone 6001, IHC, WB) LS‑C355408

Submit Review LSBio Guarantee
Antibody:
APOA1 / Apolipoprotein A 1 Mouse anti-Human Monoclonal (6001) Antibody
Application:
IHC, WB, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$857
LS-C355408-1
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
APOA1 / Apolipoprotein A 1 Mouse anti-Human Monoclonal (6001) Antibody
Application:
IHC, WB, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Apolipoprotein A 1 antibody LS-C355408 is an unconjugated mouse monoclonal antibody to human Apolipoprotein A 1 (APOA1). Validated for ELISA, IHC, RIA and WB.
Target
Human APOA1 / Apolipoprotein A 1
Synonyms
APOA1 | ApoA-I | APOAI | Apolipoprotein A-I | Apolipoprotein A1 | Apo-AI
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
6001
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Immunogen
Purified human plasma Apo A-1.
Specificity
The antibody targets Apolipoprotein A-1 in ELISA and RIA applications and shows reactivity with Human samples. It detects Apolipoprotein A-1 which has a predicted molecular weight of approximately 29 kD.
Applications
  • IHC
  • Western blot
  • ELISA
  • Radioimmunoassay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.015 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APOA1 / Apolipoprotein A 1
P02647 NM_000039 NP_000030.1

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APOA1 / Apolipoprotein A 1 Antibody - Anti-Apolipoprotein A I antibody IHC of mouse liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC‑plus™ APOA1 / Apolipoprotein A 1 Antibody LS‑B5257
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APOA1 / Apolipoprotein A 1 Antibody - Anti-Apolipoprotein A I antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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See all APOA1 / Apolipoprotein A 1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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