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Anti-Apg7 / ATG7 Antibody (N-Terminus) IHC-plus™ LS-B2195

Note: This antibody replaces LS-C19539
Catalog Size Price
LS-B2195-50 50 µg (1 mg/ml) Unavailable

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100% Guaranteed
Rabbit Polyclonal (IgG) to Human Apg7 / ATG7
Human, Mouse
IHC, IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human Apg7 / ATG7
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (1 - 2 µg/ml)

Specificity and Use

LS-E30180 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B2195. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
N-Terminus
Immunohistochemistry: LS-B2195 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2195 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Store at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze thaw cycles.
For research use only.

About Apg7 / ATG7

O95352 NM_006395 NP_006386.1

ATG7 Antibody, Apg7 Antibody, APG7L Antibody, APG7-LIKE Antibody, Autophagy related 7 Antibody, Autophagy-related protein 7 Antibody, HAGP7 Antibody, GSA7 Antibody

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition.

Immunohistochemistry

Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2195 concentration 5 ug/ml.
Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2195 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of APG7 in L1210 cells with APG7 antibody at 10 ug/ml.
Immunocytochemistry of APG7 in L1210 cells with APG7 antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of APG7 in L1210 cells with APG7 antibody at 10 ug/ml.
Immunofluorescence of APG7 in L1210 cells with APG7 antibody at 10 ug/ml.

Western blot

Western blot analysis of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.
Western blot analysis of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.

Western blot

Western blot analysis of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.
Western blot analysis of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.

Western blot

Western blot of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.
Western blot of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.

Western blot

Western blot analysis of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.
Western blot analysis of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.

Requested From: 
Date Requested: 4/22/2018

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