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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Apg7 / ATG7 Antibody (N‑Terminus) IHC‑plus™ LS‑B2217
Note: This antibody replaces LS-C3768
ATG7 antibody LS-B2217 is an unconjugated rabbit polyclonal antibody to ATG7 (Apg7) from human and mouse. Validated for ELISA, ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2217-50
50 µg
Unavailable

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Product Description

ATG7 antibody LS-B2217 is an unconjugated rabbit polyclonal antibody to ATG7 (Apg7) from human and mouse. Validated for ELISA, ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About Apg7 / ATG7
E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. O95352 NM_006395 NP_006386.1

ATG7 Antibody, Apg7 Antibody, APG7L Antibody, APG7-LIKE Antibody, Autophagy related 7 Antibody, Autophagy-related protein 7 Antibody, HAGP7 Antibody, GSA7 Antibody


Specifications

Target
Human Apg7 / ATG7
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC (10 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Apg7 / ATG7 antibody was raised against a 17 amino acid peptide from near the amino terminus of human APG7 (Genbank accession No. NP_006386).
Epitope
N-Terminus
Specificity
Recognizes human APG7.
Usage
Immunohistochemistry: LS-B2217 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2217 was determined to be 5 ug/ml. Positive control: L1210 cell lysate.
Presentation
PBS, pH 7.2, 0.02% sodium azide.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2217 concentration 5 ug/ml.
Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2217 concentration 5 ug/ml.

Immunohistochemistry

Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2217 concentration 5 ug/ml.
Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2217 concentration 5 ug/ml.

Immunohistochemistry

Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2217 concentration 5 ug/ml.
Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2217 concentration 5 ug/ml.

Immunohistochemistry

Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2217 concentration 5 ug/ml.
Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2217 concentration 5 ug/ml.


Requested From: 
Date Requested: 6/18/2018

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