(tested or 100% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- ICC (10 µg/ml)
- Western blot
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Apg7 / ATG7 antibody was raised against a 17 amino acid peptide from near the amino terminus of human APG7 (Genbank accession No. NP_006386).
Recognizes human APG7.
Immunohistochemistry: LS-B2217 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2217 was determined to be 5 ug/ml. Positive control: L1210 cell lysate.
PBS, pH 7.2, 0.02% sodium azide.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.
This antibody carries the LSBio 100% Guarantee.