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Anti-APG5 / ATG5 Antibody (C-Terminus) IHC-plus™ LS-B4678

Note: This antibody replaces LS-C81824

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular APG5 / ATG5 Antibodies

Anti-APG5 / ATG5 Antibody IHC-plus™ LS-B1843
Rabbit Polyclonal to Human APG5 / ATG5
Human, Mouse, Rat, Bovine, Pig, Pufferfish, Zebrafish
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-APG5 / ATG5 Antibody (C-Terminus) IHC-plus™ LS-B4678
Rabbit Polyclonal (IgG) to Human APG5 / ATG5
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Rabbit, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human APG5 / ATG5
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Rabbit, Xenopus
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human APG5 / ATG5
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Rabbit, Xenopus (tested or 100% immunogen sequence identity)
Chicken, Zebrafish, Drosophila (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

APG5 / ATG5 antibody was raised against synthetic peptide from C-Terminus of human ATG5 (Q9H1Y0, NP_004840). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Xenopus, Catfish, Beetle (100%); Turkey, Zebra finch, Sparrow, Chicken, Lizard, Salmon, Stickleback, Medaka, Pufferfish, Zebrafish, Drosophila (92%).
LS-E19517 - Lyophilized - 100 µg - $145.00
C-Terminus
Human ATG5
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About APG5 / ATG5

Q9H1Y0 NM_004849 NP_004840.1

ATG5 Antibody, APG5 Antibody, APG5-LIKE Antibody, APG5L Antibody, Apoptosis specific protein Antibody, Apoptosis-specific protein Antibody, Autophagy related 5 Antibody, HAPG5 Antibody, ASP Antibody, Autophagy protein 5 Antibody

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity.

Immunohistochemistry

Immunohistochemistry
Anti-ATG5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4678 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-ATG5 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4678 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/8/2016

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