LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All ELISA Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-APG5 / ATG5 Antibody (aa209-238) LS-C156610

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular APG5 / ATG5 Antibodies

Anti-APG5 / ATG5 Antibody IHC-plus™ LS-B1843
Rabbit Polyclonal to Human APG5 / ATG5
Human, Mouse, Rat, Bovine, Pig, Pufferfish, Zebrafish
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-APG5 / ATG5 Antibody (C-Terminus) IHC-plus™ LS-B4678
Rabbit Polyclonal (IgG) to Human APG5 / ATG5
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Rabbit, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human APG5 / ATG5
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human APG5 / ATG5
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)

Specificity and Use

LS-E5603 - Lyophilized - 100 µg - $145.00
aa209-238
This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-238 amino acids from the C-terminal region of human ATG5.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About APG5 / ATG5

Q9H1Y0 NM_004849 NP_004840.1

ATG5 Antibody, APG5 Antibody, APG5-LIKE Antibody, APG5L Antibody, Apoptosis specific protein Antibody, Apoptosis-specific protein Antibody, Autophagy related 5 Antibody, HAPG5 Antibody, ASP Antibody, Autophagy protein 5 Antibody

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence

Immunofluorescence
Fluorescent image of U251 cells stained with ATG5 Antibody. Antibody was diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 mu M, 16h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot
Western blot of APG5L (arrow) using rabbit polyclonal APG5L Antibody (P224). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene.

Western blot

Western blot
Western blot of APG5L antibody in Y79 cell line, mouse liver tissue, and HeLa cell line lysates.

Western blot

Western blot
Cos7, HEK293, MEF, and HeLa cells left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.
 

Requested From: United States
Date Requested: 2/20/2017

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2017 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number