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Anti-APG12 / ATG12 Antibody (Internal) IHC-plus™ LS-B2140

Note: This antibody replaces LS-C11938


Wt. Vol. Conc. Price
50 µg - 0.25 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular APG12 / ATG12 Antibodies

Anti-APG12 / ATG12 Antibody (N-Terminus) LS-C53240
Rabbit Polyclonal to Human APG12 / ATG12
Human, Mouse, Rat
Western blot
Immunofluorescence Image
Anti-APG12 / ATG12 Antibody (Internal) IHC-plus™ LS-B3216
Rabbit Polyclonal to Human APG12 / ATG12
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-APG12 / ATG12 Antibody LS-C156567
Mouse Monoclonal (IgG2b,k) to Human APG12 / ATG12
IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human APG12 / ATG12
Human, Mouse
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA


Human APG12 / ATG12
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)

Specificity and Use

APG12 / ATG12 antibody was raised against synthetic peptide derived from the internal region of the human Apg12 protein.
Recognizes human Apg12 protein at ~22kD, as well as additional bands between 40 and 80kD of unknown origin. Reactivity has been confirmed with Apg12-transfected, overexpressed HEK293 cell lysates.
Immunohistochemistry: LS-B2140 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2140 was determined to be 5 ug/ml. Immunoprecipitation: 7 ug/reaction.


PBS, pH 7.4, 0.1% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About APG12 / ATG12

O94817 NM_004707 NP_004698.3

ATG12 Antibody, Autophagy-related protein 12 Antibody, APG12L Antibody, Autophagy related 12 Antibody, FBR93 Antibody, APG12 Antibody, APG12-like Antibody, Ubiquitin-like protein ATG12 Antibody, HAPG12 Antibody

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.


Anti-ATG12 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2140 concentration 5 ug/ml.

Requested From: United States
Date Requested: 10/25/2016

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