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Anti-APG12 / ATG12 Antibody (C-Terminus) LS-C117261

Ordering

Wt. Vol. Conc. Price
50 µg - - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular APG12 / ATG12 Antibodies

Anti-APG12 / ATG12 Antibody (N-Terminus) LS-C53240
Rabbit Polyclonal to Human APG12 / ATG12
Human, Mouse, Rat
Western blot
Unconjugated
Immunofluorescence Image
Anti-APG12 / ATG12 Antibody (Internal) IHC-plus™ LS-B3216
Rabbit Polyclonal to Human APG12 / ATG12
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-APG12 / ATG12 Antibody LS-C156567
Mouse Monoclonal (IgG2b,k) to Human APG12 / ATG12
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human APG12 / ATG12
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human APG12 / ATG12
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

APG12 / ATG12 antibody was raised against synthetic peptide from C-Terminus of human ATG12 (O94817, NP_004698). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Xenopus, Zebrafish (100%); Stickleback (92%).
LS-E19516 - Lyophilized - 100 µg - $145.00
C-Terminus
Human ATG12
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About APG12 / ATG12

O94817 NM_004707 NP_004698.3

ATG12 Antibody, Autophagy-related protein 12 Antibody, APG12L Antibody, Autophagy related 12 Antibody, FBR93 Antibody, APG12 Antibody, APG12-like Antibody, Ubiquitin-like protein ATG12 Antibody, HAPG12 Antibody

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.

Western blot

Western blot
ATG12 antibody LS-C117261 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/3/2016

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