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Anti-APAF1 / APAF-1 Antibody (aa289-338) IHC-plus™ LS-B7591

Note: This antibody replaces LS-C118390
Catalog Size Price
LS-B7591-50 50 µl Unavailable

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Immunofluorescence of Apaf1 in K562 cells with Apaf1 antibody at 10 ug/ml.
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Formalin-fixed, paraffin-embedded tissue sections of human brain tumors stained for Apaf 1 expression using LS-C148201 at 1:2000. A. Gemistocytoma (grade II). B. Anaplastic glioma. A high level of Apaf 1was observed in the gemistocytoma whereas a low level was seen in the more malignant anaplastic glioma. Hematoxylin-Eos in counterstain.
Anti-APAF1 / APAF-1 Antibody (aa1-464, clone 2E12, Low Endotoxin) IHC-plus™ LS-B12884
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Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unmodified form of this product. Other forms have not been tested.

100% Guaranteed
Rabbit Polyclonal (IgG) to Human APAF1 / APAF-1
Human
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated

Details

Human APAF1 / APAF-1
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:10000)

Specificity and Use

APAF1 / APAF-1 antibody was raised against synthetic peptide from human APAF-1-ALT (aa289-338).
aa289-338
APAF-1-ALT Antibody detects endogenous levels of total APAF-1-ALT protein.

Packaging

PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Store at -20°C for up to 1 year.
For research use only.

About APAF1 / APAF-1

O14727 NM_013229 NP_037361.1

APAF1 Antibody, APAF-1 Antibody, CED4 Antibody, KIAA0413 Antibody

Apaf1 has been described as the core of the apoptosome. Deficiency in murine Apaf1 leads to embryonic lethality with a phenotype affecting many aspects of developmental apoptosis. In the developing brain, Apaf1 is a death regulator of the neuronal founder cells. Combined intercrosses of mouse lines mutant for members of the mitochondrial death pathway are providing us with some clues about the relative regulation existing among neuronal cell populations.

Immunohistochemistry

Anti-APAF1 / APAF-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7591 dilution 1:100.
Anti-APAF1 / APAF-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7591 dilution 1:100.

Western blot

Western blot of extracts from COLO205 cells, using APAF-1-ALT Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from COLO205 cells, using APAF-1-ALT Antibody. The lane on the right is treated with the synthesized peptide.

Requested From: 
Date Requested: 5/27/2018

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