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Anti-AP2A1 / AP2-Alpha Antibody (aa706-727) IHC-plus™ LS-B9566

Note: This antibody replaces LS-C186441

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular AP2A1 / AP2-Alpha Antibodies

Anti-AP2A1 / AP2-Alpha Antibody (clone AC1-M11) LS-C20294
Mouse Monoclonal [clone AC1-M11] (IgG2a) to Bovine AP2A1 / AP2-Alpha
Bovine, Human, Mouse, Rat, Pig, Chicken
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Anti-AP2A1 / AP2-Alpha Antibody (aa612-661) LS-C135681
Rabbit Polyclonal to Human AP2A1 / AP2-Alpha
Human, Monkey, Mouse, Rat, Guinea pig, Horse, Rabbit
Western blot
Unconjugated
Western blot Image
Anti-AP2A1 / AP2-Alpha Antibody (aa706-727) IHC-plus™ LS-B9566
Goat Polyclonal to Human AP2A1 / AP2-Alpha
Human, Monkey, Mouse, Dog, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human AP2A1 / AP2-Alpha
Human, Monkey, Mouse, Dog, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human AP2A1 / AP2-Alpha
Goat
Human, Monkey, Mouse, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.01 - 0.03 µg/ml)
  • ELISA (1:128000)

Specificity and Use

AP2A1 / AP2-Alpha antibody was raised against synthetic peptide C-ELEPPAPESPMALLADPAPAAD from an internal region of human AP2A1 / AP2-Alpha (NP_055018.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Ferret, Dog, Cat, Panda, Horse, Rabbit, Pig (100%); Rat (95%); Opossum, Guinea pig (91%).
LS-E28012 - Lyophilized - 100 µg - $145.00
aa706-727
Human AP2A1 / AP2-Alpha. This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 110kD band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kD according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Recommended concentration: 0.01-0.03 ug/ml. Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About AP2A1 / AP2-Alpha

O95782 NM_014203 NP_055018.2

AP2A1 Antibody, Adaptin, alpha A Antibody, ADTAA Antibody, Alpha-adaptin A Antibody, Alpha1-adaptin Antibody, AP-2 complex subunit alpha-1 Antibody, AP2 Alpha Antibody, CLAPA1 Antibody, AP2-alpha Antibody

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation.

Immunohistochemistry

Immunohistochemistry
Anti-AP2A1 / AP2-Alpha antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9566 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry
AP2A1 antibody (0.1 ug/ml) staining of methanol-fixed HeLa cells with stably expressing Mouse Ap2a1. Primary incubation was 1 hour. Detected by Alexa Fluor 594. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK

Western blot

Western blot
AP2A1 antibody (0.01 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Western blot
AP2A1 antibody (0.1 ug/ml) staining of Mouse Brain lysate (~5 ug protein in SDSPAGE buffer). The left panel shows the same blot stained with Ponceau red for total protein stain as the loading control before labeling. Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK

Requested From: United States
Date Requested: 12/10/2016

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