Human AP1G1 / Adaptin Gamma 1

Goat

Human (tested or 100% immunogen sequence identity)

Chimpanzee, Orangutan, Mouse, Rat, Dog, Chicken (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 625-675 of Human AP1G1.

aa625-675

A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and orangutan based on 100% homology for the immunogen sequence. Cross reactivity with AP1G1 protein from mouse, rat, dog and chicken may occur as sequence homology varies by only one amino acid residue in this sequence as indicated by BLAST analysis. Reactivity with AP1G1 proteins from other sources is not known.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot (1:500 - 1:2000)
• Immunoprecipitation (1:100)
• ELISA (1:2000 - 1:8000)

Immunohistochemistry: LS-B344 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B344 was determined to be 2.5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O43747 NCBI: NM_001128 NP_001119.3