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Anti-ANXA5 / Annexin V Antibody (clone RUU-WAC2A) LS-C146643

Ordering

Wt. Vol. Conc. Price
100 µg - - $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular ANXA5 / Annexin V Antibodies

Anti-ANXA5 / Annexin V Antibody (clone 1F4-1A5) IHC-plus™ LS-B5377
Mouse Monoclonal [clone 1F4-1A5] (IgG1,k) to Human ANXA5 / Annexin V
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ANXA5 / Annexin V Antibody (aa1-320, Biotin) LS-C297780
Rabbit Polyclonal (IgG) to Human ANXA5 / Annexin V
Human
Western blot, ELISA
Biotin Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone RUU-WAC2A] (IgG1) to Human ANXA5 / Annexin V
Human
IHC - Frozen, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human ANXA5 / Annexin V
Mouse
Human (tested or 100% immunogen sequence identity)
Monkey, Rat
IgG1 Monoclonal [RUU-WAC2A]
Unconjugated
Purified
Unmodified

Applications

  • IHC - Frozen (1:50 - 1:100)
  • Immunofluorescence
  • Western blot (1:100 - 1:500)
  • ELISA

  • IHC - Paraffin

Specificity and Use

ANXA5 / Annexin V antibody was raised against recombinant human annexin A5
Specifically reacts with annexin A5; directed to the phospholipid binding domain of annexin A5, and as such it inhibits annexin A5 binding to anionic phospholipid membranes, phospholipid-containing vesicles, activated platelets and apoptotic cells for more than 95%. Does not remove annexin A5 bound to phospholipid surfaces, indicating that the epitope is not accessible after interaction with annexin A5. It shows no cross reactivity with Annexins I, II, III, IV, VI, VII and VIII in ELISA or in immunoprecipitation. It does not recognize monkey and rat Annexin V in ELISA.
For immuno-histochemistry of paraffin sections, a pretreatment step with 2% amino-propyl-triethoxysilan (in 100% ethanol) and 0.2 % BSA is recommended. The sections are heated for 15 minutes at 100°C in a citrate buffer (10 mM trisodium citrate, pH 6,0).

Packaging

PBS containing 0.09% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About ANXA5 / Annexin V

P08758 NM_001154 NP_001145.1

ANXA5 Antibody, ANX5 Antibody, Anchorin CII Antibody, Calphobindin I Antibody, CBP-I Antibody, ENX2 Antibody, PP4 Antibody, RPRGL3 Antibody, Lipocortin V Antibody, Thromboplastin inhibitor Antibody, VAC-alpha Antibody, Vascular anticoagulant-alpha Antibody, PAP-I Antibody, Annexin A5 Antibody, Annexin V Antibody, Annexin-5 Antibody, Endonexin II Antibody

ANXA5 / Annexin V belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation.

Immunohistochemistry

Immunohistochemistry
Immunofluorescence staining of a 7 days old zebrafish embryo
 

Requested From: United States
Date Requested: 12/9/2016

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