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Anti-ANXA1 / Annexin A1 Antibody (aa324-337) IHC-plus™ LS-B3028

Note: This antibody replaces LS-C87404
Catalog Size Price
LS-B3028-50 50 µg (0.5 mg/ml) Unavailable

Most Popular ANXA1 / Annexin A1 Antibodies

Anti-ANXA1 / Annexin A1 Antibody (N-Terminus) LS-C30822
Rabbit Polyclonal (IgG) to Human ANXA1 / Annexin A1
Human, Mouse, Bovine, Dog, Guinea pig, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ANXA1 / Annexin A1 Antibody (aa324-337) IHC-plus™ LS-B3028
Goat Polyclonal to Human ANXA1 / Annexin A1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ANXA1 / Annexin A1 Antibody (N-Terminus) IHC-plus™ LS-B3363
Rabbit Polyclonal to Human ANXA1 / Annexin A1
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-ANXA1 / Annexin A1 Antibody (clone 3A8) LS-C115019
Mouse Monoclonal [clone 3A8] (IgG1) to Human ANXA1 / Annexin A1
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ANXA1 / Annexin A1 Antibody IHC-plus™ LS-B6711
Rabbit Polyclonal (IgG) to Human ANXA1 / Annexin A1
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed Publications (1)
Goat Polyclonal to Human ANXA1 / Annexin A1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human ANXA1 / Annexin A1
Goat
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (4 µg/ml)
  • Western blot (0.5 - 1.5 µg/ml)
  • ELISA (1:32000)

Specificity and Use

Synthetic peptide CQAILDETKGDYEK from the C-terminus of human ANXA1 / Annexin A1 (NP_000691.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Guinea pig (100%); Platypus (93%); Lizard (86%).
LS-E26990 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3028. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa324-337
Human ANXA1 / Annexin A1.
Immunohistochemistry: LS-B3028 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3028 was determined to be 4 ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 35kD band observed in Human lysates of cell lines A431 and K562 (calculated MW of 38.7kD according to NP_000691.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paweletz et al, Cancer Res. 2000 Nov15;60(22):6293-7. PMID: 11103786). Recommended concentration: 0.5-1.5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

Publications (1)

1
Exposure to chemical cocktails before or after conception – The effect of timing on ovarian development. Bellingham M, Amezaga MR, Mandon-Pepin B, Speers CJ, Kyle CE, Evans NP, Sharpe RM, Cotinot C, Rhind SM, Fowler PA. Molecular and cellular endocrinology. 2013 Aug;376:156-72. (WB; Sheep) [Full Text Article] [PubMed:23791816]

About ANXA1 / Annexin A1

P04083 NM_000700 NP_000691.1

ANXA1 Antibody, Annexin A1 Antibody, Annexin I Antibody, Annexin-1 Antibody, ANX1 Antibody, Calpactin II Antibody, Chromobindin-9 Antibody, Lipocortin I Antibody, Annexin I (lipocortin I) Antibody, Calpactin-2 Antibody, LPC1 Antibody

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35000 to 40000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kD, with phospholipase A2 inhibitory activity.

Immunohistochemistry

Immunohistochemistry
Anti-Annexin A1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3028 concentration 4 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-Annexin A1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3028 concentration 4 ug/ml.

Western blot

Western blot
Antibody (0.5 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 8/20/2017

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