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Anti-ANTXR1 / TEM8 Antibody LS-C150083

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ANTXR1 / TEM8 Antibodies

Anti-ANTXR1 / TEM8 Antibody (Internal) LS-C425
Rabbit Polyclonal to Human ANTXR1 / TEM8
Human, Mouse, Rat
IHC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ANTXR1 / TEM8 Antibody (C-Terminus) LS-C426
Rabbit Polyclonal to Human ANTXR1 / TEM8
Human, Mouse, Rat
ICC, Western blot
Unconjugated
Immunocytochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human ANTXR1 / TEM8
Human
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human ANTXR1 / TEM8
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • ICC (5 - 15 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

ANTXR1 / TEM8 antibody was raised against e. coli-derived recombinant human TEM8/ANTXR1 Gln28-Gly320 Accession # Q9H6X2.
Detects human TEM8/ANTXR1 in direct ELISAs and Western blots.
Western Blot, 1 µg/ml. Immunocytochemistry, 5-15 µg/ml. Immunohistochemistry, 5-15 µg/ml, Immersion fixed paraffin-embedded sections of human colon cancer.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About ANTXR1 / TEM8

Q9H6X2 NM_018153 NP_060623.2

ANTXR1 Antibody, Tumor endothelial marker 8 Antibody, Anthrax toxin receptor 1 Antibody, TEM8 Antibody

ANTXR1 / TEM8 is a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells.

Immunocytochemistry

Immunocytochemistry
TEM8 in HT-29 Human Cell Line. TEM8 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Goat Anti-Human TEM8 Isoform 1 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (yellow) and counterstained with DAPI (blue).

Western blot

Western blot
Detection of Human TEM8 Isoform 1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human TEM8 Isoform 1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. Specific bands were detected for TEM8 at approximately 55 and 45 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: United States
Date Requested: 12/7/2016

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