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Anti-ANAPC1 / APC1 Antibody (phospho-Ser377) IHC-plus™ LS-B68

Note: This antibody replaces LS-C18996

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular ANAPC1 / APC1 Antibodies

Anti-ANAPC1 / APC1 Antibody (phospho-Ser377) IHC-plus™ LS-B68
Rabbit Polyclonal to Human ANAPC1 / APC1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ANAPC1 / APC1 Antibody (C-Terminus) IHC-plus™ LS-B6979
Rabbit Polyclonal to Human ANAPC1 / APC1
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ANAPC1 / APC1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human ANAPC1 / APC1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:200 - 1:1000)
  • ELISA (1:2000 - 1:10000)

Specificity and Use

ANAPC1 / APC1 antibody was raised against synthetic peptide from human ANAPC1.
pSer377
Amino acids 373-382 of Human Apc1 protein.
Immunohistochemistry: LS-B68 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B68 was determined to be 10 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About ANAPC1 / APC1

Q9H1A4 NM_022662 NP_073153.1

ANAPC1 Antibody, APC1 Antibody, Cyclosome subunit 1 Antibody, MCPR Antibody, Mitotic checkpoint regulator Antibody, TSG24 Antibody

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Immunohistochemistry

Immunohistochemistry
Anti-ANAPC1 antibody IHC of human pancreatic carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B68 concentration 10 ug/ml.

Western blot

Western blot
Anti-APC1 pS377 Antibody - Western Blot. Western blot of Affinity Purified anti-APC1 pS377 antibody shows detection of a band ~215 kD corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. While some phosphorylated APC1 is present in untreated cell, the amount of phosphorylated protein is increased in cell preparations arrested in mitosis. Each lane contains approximately 30 ug of HeLa whole cell lysates, separated by 4-8% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000 overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 12/6/2016

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