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Anti-ANAPC1 / APC1 Antibody (phospho-Ser355) LS-C18983

Catalog Size Price
LS-C18983-100 100 µg Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human ANAPC1 / APC1
Human, Mouse, Rat, Bovine, Dog
Western blot, ELISA
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Details

Human ANAPC1 / APC1
Rabbit
Human, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:200 - 1:2000)
  • ELISA (1:10000 - 1:40000)

Specificity and Use

ANAPC1 / APC1 antibody was raised against synthetic peptide from human ANAPC1.
pSer355
Amino acids 351-359 of Human Apc1 protein.
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band - 215 kD in size corresponding to APC1 by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About ANAPC1 / APC1

Q9H1A4 NM_022662 NP_073153.1

ANAPC1 Antibody, APC1 Antibody, Cyclosome subunit 1 Antibody, MCPR Antibody, Mitotic checkpoint regulator Antibody, TSG24 Antibody

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Western blot

Western blot
Anti-APC1 pS355 Antibody - Western Blot. Western blot of Affinity Purified anti-APC1 pS355 antibody shows detection of a band ~215 kD corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with thymidine to synchronize cells at the G1/S boundary. Lane 3 shows lysate from cells treated with nocodazole to synchronize cells at the M phase. Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division. Each lane contains approximately 30 ug of HeLa S3 whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose. After blocking with 5% non-fat dry milk in TTBS, the membrane was probed with the primary antibody diluted to 1:500 for 1 h at room temperature followed by washes and reaction with a 1:5000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX (LS-C60865) for 45 min at room temperature. ECL reagent was used for detection. Other detection systems will yield similar results. Data contributed by Bing Li, UT Southwestern.

Requested From: 
Date Requested: 10/22/2017

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